rev:   October 19, 2004

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MOUSE/RAT Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:   recombinant MURINE IP-10

-Murine IP-10 is a protein produced by several cell types uring the delayed type hypersentivity response. IP-10 acts as a chemoattractant towards monocytes, lymphocytes, and certain T cells. This rec murine IP-10 is a 8.7 kDa protein containing 77 amino acid residues.

Catalog#: RDI-2516

Package Size: 20 micrograms $231.00     $219.00/package 2+

                      Mini-Pack:cat#RDI-2516P $94.00/5ug             -inquire for custom sizes/bulk packaging

                     -Bulk Quotes upon request

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of IP-10

Reconstitution: The lyophilized murine IP-10 should be reconstituted in water to a concentration of 0.1- 1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted IP-10 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The reactivity of this rec murine IP-10 was determined by a chemotaxis assay using IL-2 activated T cells. Significant chemotaxis was achieved using a concentration of 0.1-10ng/ml.

AA Sequence:

IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE

IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federalrules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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