rev::  October 19, 2004

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MOUSE/RAT Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant MURINE MIP-2

-Murine MIP-2 promotes neutrophil chemotaxis and degranulation. This rec murine MIP-2 is a 7.8 kDa protein containing 73 amino acid residues.

Package Size: 20 micrograms $231.00     $219.00/package 2+

                       Mini-Pack: cat#RDI-2515P $94.00/5ug           -inquire for custom sizes/bulk packaging

                     -Bulk and specialty packaging Quotes upon request


Supplied: Lyophilized powder from 10mM HoAc with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of MIP-2

Reconstitution: The lyophilized MIP-2 should be reconstituted in water to a concentration of 0.1- 1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEg C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEg C. Reconstituted MIP-2 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The reactivity of this rmurine MIP-2 was determined by a chemotaxis assay . Significant chemotaxis on total human neutrophils was achieved using a concentration of 0.1-10ng/ml.

AA Sequence :

AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV

IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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