rev: October 19, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
-murine MCP-2 (Monocyte Chemotactic Protein-2) plays an important role in inflammatory response of blood monocytes. This recombinant murine MCP-2 is an 8.5kDa protein containing 74 amino acid residues.
Catalog#: RDI-2514
Package Size: 20 micrograms $231.00/vial $219.00/package 2+ BULK ON
REQUEST
Mini-Pack: cat#RDI-2514P $94.00/5ug -inquire for custom sizes/bulk packaging
Supplied: Lyophilized protein with no additives.
Source: E. Coli
Purity: >99% by SDS-Page analysis and HPLC analysis. Endotoxin level is
less than 0.1ng per ug (1EU/ug) of murine MCP-2
Reconstitution: The lyophilized MCP-2 is soluble in water and most aqueous
buffers. The lyophilized powder should be reconstituted in water or buffer
to a concentration of 0.1-1mg/ml. Let sit at least 30 minutes, tighten cap,
rotate vial to reconstitute any material on sides of vial. If desired, you
can centrifuge the vial for 1-2 minutes at 1000 rpm to concentrate material
in bottom of vial. This material can be diluted into water or other buffered
solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future
use. It is recommended that further dilutions of the stock be made into buffer
containing carrier protein or media containing serum.
Storage: The lyophilized powder is stable at room temperature for a few weeks
but it is best stored desiccated at -20 DEG C. Reconstituted MCP-2 should
be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze- THAW
Cycles.
Reactivity: The murine MCP-2 biological activity was determined by its ability
to chemoattract human peripheral blood monocytes using a concentration of
10- 100ng/ml.
AA Sequence:
GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV
FQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQP
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user to comply
with all local/state and Federal rules in the use of this product. We are
not responsible for any patent infringements that might result with the use
of or derivation of this product.
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cytokine index)
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