rev: October 19, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
(murine Macrophage Inflammatory Protein 1 gamma)
-murine Macrophage inflammatory protein 1 gamma is a monokine with inflammatory,
pyrogenic and chemokinetic CC properties. When murine MIP-1 gamma binds to
a high affinity receptor it activates CC calcium release in neutrophils.
It also inhibits colony formation CC of bone marrow myeloid immature progenitors.
This rec mMIP-1g is an 11.6kDa protein containing 101 amino acid residues.
Catalog#: RDI-2512
Package Size: 20 micrograms $231.00/vial $219.00/package
2+ BULK ON REQUEST
Mini-Pack:cat#RDI-2512P $94.00/5ug
Supplied: Lyophilized protein with no additives.
Source: E. Coli
Purity: >99% by SDS-Page analysis and HPLC analysis. Endotoxin level is
less than 0.1ng per ug of MIP-1gamma
Reconstitution: The lyophilized MIP-g is soluble in water and most aqueous
buffers. The lyophilized powder should be reconstituted in water to a
concentration of 1mg/ml. Let sit at least 30 minutes, tighten cap, rotate
vial to reconstitute any material on sides of vial. If desired, you can
centrifuge the vial for 1-2 minutes at 1000 rpm to concentrate material in
bottom of vial. This material can be diluted into water or other buffered
solutions or stored at - 20 DEG C for future use. It is recommended that
further dilutions of the stock be made into buffer containing carrier protein
or media containing serum.
Storage: The lyophilized powder, though stable at room temperature, is best
stored desiccated below 0 DEG C. Reconstituted MIP-1 gamma should be stored
in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
Reactivity: The murine MIP-1g biological activity was determined by a chemotaxis
assay. Significant chemotaxis was achieved at a concentration of 100ng/ml.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user to comply
with all local/state and Federal rules in the use of this product. We are
not responsible for any patent infringements that might result with the use
of or derivation of this product.
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