rev: October 19, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
-Description:Murine KC also known as Murine Gro-Alpha belongs to the C-X-C family of chemokines. This rec murine KC is a 7.8 kDa protein containing 72 amino acid residues
Catalog#: RDI-2511
Package Size: 20 micrograms $235.00 $219.00/package 2+
Mini-Pack:cat#RDI-2511P $94.00/5ug
-Bulk Quotes upon request
-inquire for custom
sizes/bulk packaging
Supplied: Sterile filtered and then lyophilized powder with no additives.
Source: E. Coli
Purity: >99% by SDS-Page and HPLC analysis Endotoxin level is less than
0.1ng per ug (1EU/mg) of Murine KC
Reconstitution: The lyophilized Murine KC should be reconstituted in water to a concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEg C for up to 1 week or at -20 DEG C-20 DEG C for future use.
Note:If this material is not to be used for coating or labeling or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted mKC should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The biological activity of murine KC was determined by a chemotaxis assay . The maximal chemotactic response was found to be 10-100ng/ml.
AA Sequence:
APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV
IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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