rev: January 11, 2005
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
IL-7 is a hematopoietic growth factor which affects primarily early B and
T cells. Produced by thymic stromal cells, spleen cells and keratinocytes,
IL-7 can also co-stimulate the proliferation of mature T cells in combination
with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species
reactive. Recombinant murine IL-7 is a 15.0 kDa protein containing 130 amino
acid residues.
Catalog#: RDI-2177
Package Size: 10 micrograms $231.00 $219.00/package 2 or more
Mini-PacK; cat#RDI-2177P $94.00/2ug
-inquire for custom sizes/bulk
packaging
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin level is
less than 0.1ng per ug (1EU/ug) of IL-7.
Reconstitution: The lyophilized IL-7 should be reconstituted in water to
a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG
C , tighten cap and rotate to reconstitute any material on sides of vial.
If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume
in bottom of vial.This solution can be diluted into other buffered solutions
or stored at 4 DEG C for up to 1 week or aliquoted and stored at -20 DEG
C for future use. For most in- vitro applications, IL-7 exerts its biological
activity in the range of 0.1 to 10.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other
applications requiring carrier free material), the addition of 0.1% BSA or
dilution in sterile culture media is recommended to enhance stability and
minimize absorption to vial. Recommend maintaining stock >10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks
but it is best stored desiccated at -20 DEG C. Reconstituted IL-7 should
be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine IXN/2B cells is <0.2ng/ml, corresponding to a specific activity of > 5MU/mg
AA Sequence:
MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN
FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL
TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT
CWNKILKGSI
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
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