rev: November 11, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-IL-6 is a pleiotropic cytokine that plays an important role in host defense
by regulating immune and inflammatory responses. Produced by T cells, monocytes,
fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological
functions. It stimulates B-cell differentiation and antibody production,
synergizes with IL-3 in megakaryocyte development and platelet production,
induces expression of hepatic acute-phase proteins, and regulates bone
metabolism. IL-6 signals through the IL-6 receptor system that consists of
two chains, IL-6R alpha and gp130. Murine IL-6 is inactive on human cells,
while both human and murine are equally active on murine cells. Recombinant
murine IL-6 is a 21.7 kDa protein containing 187 amino acid residues.
Catalog#: RDI-2166
Package Size: 10 micrograms $235.00/package $219.00/package
2 or more
Bulk quotes: email: cytokines@researchd.com
Mini-Pack: cat#RDI-2166P $94.00/2ug
Supplied: Lyophilized powder with no additives (carrier free)
Source: E. Coli
Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin level is less than 0.1ng/ug of murine IL-6 (1EU/ug)
Reconstitution: The lyophilized IL-6 MUST be reconstituted in water to a max concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, Mix well. If desired , centrifuge 1 minutes 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C (1 week) or aliquoted and stored -20 DEG C longterm. For most in-vitro applications, IL-6 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other
applications requiring carrier free material), the addition of 0.1% BSA to
dilution buffers or dilution in sterile culture media is recommended to enhance
stability and minimize absorption to vial. Ideally, maintain stock
>10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks
but is best stored desiccated at -20 DEG C. Reconstituted IL-6 should be
stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The ED50 as determined by the dose- dependent stimulation the
proliferation of IL-6 dependent murine 7TD1 cells is <0.02ng/ml,
coprresponding to a specific activity of >5 X E07 units/mg.
AA Sequence: MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV
EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG
YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ
RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE
WLRTKTIQFI LKSLEEFLKV TLRSTRQT
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
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