rev:  October 19, 2004


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET:  recombinant Human INTERLEUKIN-16    (see also 121aa form)

-Interleukin 16 (IL-16) also known as lymphocyte chemoattractant factor (LCF), stimulates a migratory response in CD4+ T cells and CD4 + monocytes and eosinophils. In addition, human IL-16 induces expression of the IL-2 receptor by T cells. IL-16 is a 13.5 kDa protein containing 130 amino acid residues.

Catalog#: RDI-216

Package Size: 10 micrograms $235.00    $219.00/pck 2+

                       Mini-Pack#cat#RDI-216P $94.00/2ug

                    -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder from 1mg/ml stock in PBS with no additives.|

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug of IL-16.

Reconstitution: Lyophilized IL-16 should be reconstituted in water to a concentration of 1 ug/ul. Allow to set at least 30 minutes, rotate or vortex mix. Centrifuge for 1-2 minutes at 1000rpm to concentrate solution in bottom of vial. This solution can be diluted into other buffered solutions or stored at -20 DEG C for future use. It is recommended that a carrier protein or medium serum be added to diluted material. For most in- vitro applications, IL-16 exerts its biological activity in the range of 0.1 to 20.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted IL-16 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: This product is fully biologically active when compared to standards. human IL-16 produced a significant increase in chemotaxis at 1.4 ng/ml compared to the medium control in an assay using human CD4 + T lymphocytes.

AA Sequence:




Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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