rev:   October 19, 2004


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET:  recombinant Human INTERLEUKIN-15

-Interleukin 15 (IL-15) is a potent T-cell growth factor whose biological activity resembles that of IL-2 and which appears to interact predominantly with the B subunit of the IL-2 receptor (IL-2RB). This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Whereas an antibody to the ALPHA subunit of the IL-2 receptor was able to block IL-2 but not IL-15 in inducing T-cell proliferation, antibodies to IL-2RB consistently inhibit IL-15 more effectively than they inhibit IL-2. Additionally, it has been shown that neutralizing antibodies to Il-2 failed to block IL-15 activity in stimulating T-cell proliferation and induction of LAK cells. These data indicate that IL-15 acts independently of IL-2 and suggest that these two cytokines can act synergistically in stimulating the cellular immune response. Human IL-15 is a 13kDa protein containing 114 amino acid residues.

Catalog#: RDI-215      LOT#03124

Package Size: 10 micrograms $231.00 or   $219.00/pack 2 or more any

                    Mini-Pack: cat#RDI-215P $94.00/2ug

                     -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder from 20mM Tris pH8.0 with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis Endotoxin less than 0.1ng per ug of IL-15.

Reconstitution: Lyophilized IL-15 should be reconstituted in water or other aqueous buffer to a maximum concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, IL-15 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder is stable for a few weeks at room temperature but it is best stored desiccated -20 DEG C. Reconstituted IL-15 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The specific activity is greater than 2 X 10 E06 units per milligram as determined by the dose dependent stimulation of CTLL-2 cell proliferation (Ed50=<0.5ng/ml, corresponding to a specific activity of >2 million units per milligram.

AA Sequence:




Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Return (human cytokine index)

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