rev: October 19, 2004
Return (RDI HOME PAGE)
Return (mouse cytokine index)
Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-Murine SF20 is a secreted bone marrow stroma-derived growth factor. SF20 is not only expressed in bone marrow and spleen stroma cells, it is expressed in resting mononuclear cells, resting CD8+ and CD19+ cells and activated CD8+ T cells. It has been shown only to bind to the surface of cells expressing the receptor TSA-1 (Thymic shared Ag-1). Reports show that SF20 stimulates the proliferation of FDCP2 (a mouse IL-3 factor-dependent hemopoietic cell line) cells and mouse lymphoid cells through TSA-1, but it does not show any myelopoietic activity. More research on SF20 and its receptor TSA-1 may help determine the role they play in the regulation of lymphocyte activation. This recombinant Murine SF20 is a 15.8 kDa protein,consisting of 143 amino acid residues.
10 micrograms $231.00 $219.00/package 2 or more (any combination)
-Mini-Pack# cat#RDI-2125p $94.00/2ug
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of SF20
Reconstitution: At first use, tighten cap, genetly tap on counter or centrifuge 1 minute 1000 rpm to concentrate powder in bottom of vial. The lyophilized SF20 should then be reconstituted isterile DI water to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for 1 week or -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to later dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at - 20 DEG C. Reconstituted SF20 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES. continued on back side
Reactivity: not determined
MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ
GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA
ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH
RPGAFKAELS KLVIVAKAAR SEL
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Return (RDI HOME PAGE)
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
all rights reserved by any/all copyright holders?