rev: October 19, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
-Description:Interleukin-2 (IL-2) is a potent lymphoid cell growth factor
which exerts its biological activity primarily on T cells. The Murine IL-2
is a 17.2 kDa protein containing 149 amino acid residues.
Catalog#: RDI-2122
Package Size: 20 micrograms $235.00 $219.00/package 2+
Mini-Pack:cat#RDI-2122P $94.00/5ug
-inquire for custom sizes/bulk
packaging
Supplied: Sterile filtered and then the protein was lyophilized from 10mM
NaCitrate pH 4.0 . lyophilized powder with no additives
Source: E. Coli
Purity: >97% by SDS-Page and HPLC analysis Endotoxin level is less than
0.1ng per ug (1EU/mg) of murine IL-2.
Reconstitution: The lyophilized IL-2 should be reconstituted in water to
a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG
C, tighten cap and rotate to reconstitute any material on sides of vial.
If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume
in bottom of vial. This solution can be diluted into other buffered solutions
containing carrier protein or medium and stored at 4 DEG C for up to 1 week
or at -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other
applications requiring carrier free material), the addition of 0.1% BSA to
dilution buffers or dilution in sterile culture media is recommended to enhance
stability and minimize absorption to vial. Ideally, maintain stock
>10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks
but it is best stored desiccated at -20 DEG C. Reconstituted MIL-2 should
be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW
CYCLES.
Reactivity: The ED(50) as determined by the dose dependent stimulation of
murine CTLL-2 cells is <0.2ng/ml, corresponding to a specific activity
of > 5 x 10(6) units/mg.
AA Sequence:
PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR
MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR
HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ
FDDESATVVD FLRRWIAFCQ SIISTSPQ
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
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