rev: November 7, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation
and proliferation of committed progenitor cells of the megakaryocyte,
granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages.
Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses,
phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to
activate monocytes suggests that IL-3 may have additional immunoregulatory
roles. Many of the IL-3 activities depend upon co-stimulation with other
cytokines. IL-3 is species-specific, variably glycosylated cytokine. This
recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino
acid residues.
Catalog#: RDI-2113
Package Size: 10 micrograms $235.00 $219.00/pack 2+
Mini-PacK; cat#RDI-2113P $94.00/5ug
-inquire for custom
sizes/bulk packaging
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page and HPLC analyses. Endotoxin level is less than
0.1ng per ug (1EU/ug) of murine IL-3.
Reconstitution: The lyophilized IL-3 should be reconstituted in sterile water
to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG
C, Mix well. If desired, centrifuge for 1 minute at 1000 rpm to concentrate
material in vial. This solution can be diluted into other buffered solutions
or stored at 4 DEG C for up to 1 week or at -20 DEG C for long term use.
For most in-vitro applications, IL-3 exerts its biological activity in the
range of 0.1 to 10.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other
applications requiring carrier free material), the addition of 0.1% BSA to
dilution buffers or dilution in sterile culture media is recommended to enhance
stability and minimize absorption to vial. Ideally, maintain stock
>10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few week
but it is best stored desiccated at -20 DEG C. Reconstituted IL-3 should
be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW
CYCLES.
AA Sequence:
MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK
SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA
NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ
PASGSVSPNR GTVEC
Reactivity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine IL-3 dependent M-NFS-60 cells is <0.-05ng/ml, corresponding to a specific activity of approx 20 million units/mg.
Old Bioassay : The ED50 as determined by the dose-dependent stimulation of the proliferation of murine IL-3 dependent FDC-P1 cells is <0.1ng/ml, corresponding to a specific activity of approx 10 million units/mg.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
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