rev: December 14, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
IL-1beta is a proinflammatory cytokine produced in a variety of cells including
monocytes, tissue macrophages, keratinocytes and other epithelial cells.
Both IL-1alpha and IL-1beta binds to the same receptor and has similar if
not identical biological properties. These cytokines have a broad range of
activities including, stimulation of thymocyte proliferation, by inducing
IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity
and the ability to stimulate the release of prostaglandin and collagenase
from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha
is predominantly a cell-associated cytokine. Recombinant murine IL-1beta
is a 17.5 kDa protein containing 153 amino acid residues.
Catalog#: RDI-2111b
Package Size: 10 micrograms $231.00 $219.00/package 2 or more
RDI-2111bP $94.00/2ug mini-pack
-inquire for custom sizes/bulk
packaging
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis and N-terminal sequence analysis. Endotoxin
level is less than 0.1 ng per ug of IL-1b.
Reconstitution: The lyophilized mIL-1b is soluble in water and most aqueous
buffers to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes
at 4 DEG C , tighten cap and rotate to reconstitute any material on sides
of vial. If desired, you can centrifuge at 1000 rpm for 1 minute concentrate
volume in bottom of vial. The lyophilized powder can be reconstituted in
water to a concentration of 0.1-1.0mg/ml. This solution can be diluted into
other buffered solutions or stored at 4 DEG C for up to 1 week or stored
at -20 DEG C for future use. For most in-vitro applications, IL-1b exerts
its biological activity in the range of 0.1 to 10.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other
applications requiring carrier free material), the addition of 0.1% BSA to
dilution buffers or dilution in sterile culture media is recommended to enhance
stability and minimize absorption to vial. Ideally, maintain stock
>50ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks
but it is best stored desiccated at -20 DEG C. Reconstituted IL-1b should
be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW
CYCLES.
Reactivity: The ED50 as determined by the dose-dependent stimulation of murine
D10S cells is <0.002ng/ml, corresponding to a specific activity of >
5 X 10 E08 units/mg.
AA Sequence:
MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ
VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT
LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN
WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.It is the responsibility of the user to comply
with all local/state and Federal rules in the use of this product. We are
not responsible for any patent infringements that might result with the use
of or derivation of this product
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