rev:    November 8, 2004


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET:recombinant Human INTERLEUKIN-4

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. This recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues

Catalog#: RDI-204  

                               $235.00/package   $219.00/package 2 or more

                            Mini-Pack: cat#RDI-204p $94.00/2ug

                            -inquire for custom sizes/bulk packaging

Package Size: 10 micrograms

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin level is less than 0.1 ng per ug (1EU/ug) of IL-4.

Reconstitution: The lyophilized IL-4 should be reconstituted in water to a maximum concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, rotate, mix well. If desired,centrifuge for 1-2 minutes at 500-1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at  4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, IL-4 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.

Note: For material that will not be used for coating or labelling (or other applications requiring carrier free material), we recommend adding 0.1% protease free BSA/HSA or media to any dilution buffers. Maintain stock concentrations of >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated below at -20 DGE C. Reconstituted IL-4 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose- dependent stimulation of human TF-1 cells is <0.2ng/ml, corresponding to a specific activity of >5 X 10 e06 units/mg.

AA Sequence:




Precautions: For In vitro research Use Only. Not for use in or on   humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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