rev: December 14, 2004
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.
Package Size: 10 micrograms $231.00/package $219.00/package 2 or more
Mini-Pack: cat#RDI-203p $94.00/2ug
See Bulk below
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis and N-terminal sequence analysis. Endotoxin
level is less than 0.1 ng per ug of IL-3.
Reconstitution: The lyophilized IL-3 should be reconstituted in sterile water to a concentration of 0.1-1.0mg/ml Allow to set at least 30 minutes at 4 DEG C, rotate/mix well. If desired, centrifuge for 1-2 minutes at 1-2K RPM to concentrate material in vial. This solution can be diluted into other buffered solutions or storedat 4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, IL-3 exerts its biological activity in the range of 0.1 to 1.0ng/ml.
Note:Note:If this material is not to be used for coating or labeling (or
other applications requiring carrier free material), the addition of 0.1%
BSA to dilution buffers or dilution in sterile culture media is recommended
to enhance stability and minimize absorption to vial.
Storage: The lyophilized powder is stable at room temperature for several weeks but it is best stored desiccated at -20 DEG C. Reconstituted IL-3 should be stored in working at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <0.1ng/ml, corresponding to a specific activity of >1 X 10 E 07 units/mg.
MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM
ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI
KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
cat# RDI-203-210X $4500.00/mg
RDI-203-210S $212.00/10ug Bulk quotes on request
Description: Recombinant Human IL-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-3 is purified by proprietary chromatographic techniques.
Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of IL-3
Formulation: The protein was lyophilized from a 1mg/ml solution in water with no additives
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Pro-Thr.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Solubility: It is recommended to reconstitute the lyophilized rHuIL-3 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized rHuIL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-3 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles.
Biological Activity: This rHuIL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less then 0.1 ng/ml, corresponding to a Specific Activity of 1 x 10 E07 IU/mg.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
For In vitro research Use Only. Not for use in or on humans or animals . It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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