rev: November 2, 2004
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant human IL-1alpha is an 18.0 kDa protein containing 159 amino acid residues.
Package Size: 10 micrograms - $231.00/package $219.00/pk 2+
Mini-Pack:cat#RDI-201aP $94.00/2ug -inquire for custom sizes/bulk packaging
Supplied: Lyophilized powder from 10mM Tris pH 8.0 + 100mM NaCl buffer with
Source: E. Coli
Purity: >98% by SDS-Page analysis HPLC analyses. Endotoxin level is less than 0.1ng/ug (1EU/ug) of IL-1 alpha.
Reconstitution: The lyophilized IL-1A is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in water to a concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, rotate, mix well. Centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use. For most in-vitro applications, IL-1A exerts its biological activity in the range of 0.1 to 10.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted IL-1A should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The ED50 as determined by the dose-dependent stimulation of murine D102 cells is <0.001ng/ml, which yield a specific activity of >1 X 10 9Units/mg.
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TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY
VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN
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Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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