rev:     November 2, 2004


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET:recombinant Human Interleukin-1 ALPHA

IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant human IL-1alpha is an 18.0 kDa protein containing 159 amino acid residues.

Catalog#: RDI-201a

Package Size: 10 micrograms - $231.00/package   $219.00/pk 2+        

                       Mini-Pack:cat#RDI-201aP $94.00/2ug         -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder from 10mM Tris pH 8.0 + 100mM NaCl buffer with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis HPLC analyses. Endotoxin level is less than 0.1ng/ug (1EU/ug) of IL-1 alpha.

Reconstitution: The lyophilized IL-1A is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in water to a concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, rotate, mix well. Centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use. For most in-vitro applications, IL-1A exerts its biological activity in the range of 0.1 to 10.0 ng/ml.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted IL-1A should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose-dependent stimulation of murine D102 cells is <0.001ng/ml, which yield a specific activity of >1 X 10 9Units/mg.

AA Sequence:





Precautions: For In vitro research Use Only. Not for use in or on humans or animals or   for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Return (human cytokine index)

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