rev:    October 19, 2004


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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET: APRIL (a proliferation-inducing Ligand), also known as TNFSF13, is a type II membrane protein belonging to the TNF Ligand family. Although low levels of APRIL are found in normal tissues, it is abundantly expressed in tumor cells. Recombinant soluble APRIL stimulates proliferation in Raji B cells, Jurkat T cells and MCF-7 carcinoma cells. Both APRIL and BAFF (BLyS) mediate their distinct biological roles through two receptors, TACI and BCMA. This Recombinant Murine APRIL is a soluble 21.9 kDa protein, consisting of 192 amino acid residues.

Catalog#: RDI-1513

Package Size: 20 micrograms $231.00    $219.00/pack 2+

                    Mini-Pack:cat#RDI-1513P $94.00/5ug

                    -inquire for custom sizes/bulk packaging

Supplied: The sterile filtered solution was lyophilized from 10 mM Tris, pH 9.0 + 100 mM Arginine.

Source: E. Coli

Purity: Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. Allow to set at least 30 minutes at 4-8 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.

Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted material should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Measured by its ability to induce cell proliferation of activated T cells

AA Sequence:

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent  infringements that might result with the use of or derivation of this product.


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