rev:    October 19, 2004

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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET: Recombinant Murine LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors; the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3). This recombinant Murine LIGHT is a soluble 20 kDa protein containing 183 amino acid residues.

Catalog#: RDI-1512 lot#

Package Size: 20 micrograms $231.00    $219.00/pack 2+

                      Mini-Pack:cat#RDI-1512P $94.00/5ug

                        -inquire for custom sizes/bulk packaging

Supplied: The sterile filtered solution was lyophilized from 20 mM Tris, pH 8.5 + 100 mM Arginine.

Source: E. Coli

Purity: Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Reconstitution: We recommend a quick spin followed by reconstitution in water or 10mM Tris pH8.5 to a concentration of 0.1-1.0 mg/ml. Allow to set at least 30 minutes at 4- 8 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.

Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted material should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent  infringements that might result with the use of or derivation of this product.

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