rev:     November 24, 2004


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HUMAN Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET:Human NOGGIN belongs to a group of structurally diverse diffusible proteins which bind TGF-beta ligands and inhibit their access to membrane receptors. This natural antagonist displays high binding-affinity for BMP-2 and -4 and a lower affinity for BMP-7 (OP-1). This protein is secreted as a disulphide-linked homodimer, however the non-disulphide-linked also assume dimeric structure and displays similar biological activity. This recombinant human NOGGIN is a 23.1 kDa non-disulphide-linked homodimer consisting of 206 amino acid residues.

Catalog#: RDI-1210

Package Size: 20 micrograms $235.00    $219.00/pack 2+

                     Mini-Pack:cat#RDI-1210P $94.00/5ug

                    -inquire for custom sizes/bulk packaging

Supplied: The sterile filtered solution was lyophilized with no additives

Source: E. coli

Purity: >95% by SDS-Page and HPLC analysis Endotoxin <1EU/ug

Reconstitution: The lyophilized human Noggin should be reconstituted in 10mM Acetic acid to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4-8 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. Note: Due to solubility reasons the protein should be kept at low pH. This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.

Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted material should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 was determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.05-0.08 µg/ml of NOGGIN.

AA Sequence:

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent  infringements that might result with the use of or derivation of this product.


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