rev: June 20, 2005
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Human Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte
cells and acts as a growth factor for a broad spectrum of tissues and cell
types. HGF signals through a transmembrane tyrosine kinase receptor known
as MET. Activities of HGF include induction of cell proliferation, motility,
morphogenesis, inhibition of cell growth, and enhancement of neuron survival.
HGF is a crucial mitogen for liver regeneration processes, especially after
partial hepatectomy and other liver injuries. Human and murine HGF are
cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide
precursor glycoprotein. Proteolytic processing of this precursor generates
the biologically active form of HGF, which consists of two polypeptide chains
(alpha-chain and beta-chain) held by a single disulfide bond resulting in
formation of a biologically active heterodimer. The alpha-chain consists
of 463 amino acid residues and four kringle domains. The beta-chain consists
of 234 amino acid residues.
Package Size: 10 micrograms $235.00 $219.00/pack 2+
-see below for bulk
Supplied: The sterile filtered solution was lyophilized from 0.1M L-Arganine
HCl + 10 mM Tris, pH 8.0.
Source: Baculovirus infected High-5 cells
Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein
Reconstitution: Lyophilized HGF should be reconstituted in water to a concentration of 0.1-0.5mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. -Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted HGF should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Description: Human Hepatocyte Growth Factor (HGF), also known as scatter factor, is a pleiotrophic cytokine that shows homology to the enzymes of the blood coagulation cascade. It stimulates the motility and invasion of several cancer cell types and can induce angiogenesis. Recently HGF was found to be identical to scatter factor, a fibroblast-derived factor promoting the dissociation of epithelial and vascular endothelial cell colonies in monolayer cell cultures by stimulating cell migration. HGF is synthesized as a biologically inactive single chain precursor, which is cleaved by a specific, extracellular serum serine protease to a fully active heterodimer. This mature, biologically active HGF consists of a disulfide-linked alpha-beta heterodimer of the two cleavage products. Previous studies have shown that single chain and heterodimeric HGF are equally active in in vitro assay systems due to either production of the serine protease in cell culture or the presence of the ubiquitious protease in serum. All biological responses induced by HGF are elicited by binding to its transmembrane tyrosine kinase receptor, which is encoded by the MET proto-oncogene. After autophosphorylation of the receptor different cytoplasmatic effectors are activated that bind to the same multifunctional docking site of the receptor. HGF function is essential for normal development. Knockout studies have demonstrated that both ligand and receptor deficient mice display an embryonic lethal phenotype. Hepatocytes have to be primed before they can fully respond to
HGF. This priming requires cytokines as TNF and IL-6. Recent studies have suggested that HGF synergizes with basic FGF in the induction of angiogenesis.
Source: nsect cells
Molecular Weight: 78.0 kDa
Specific activity: 2 x 10 6 units/mg
Purity: > 85% (SDS-PAGE, HPLC)
Endotoxin level: < 0.1 ng per ug of HGF
Buffer: 20 mM Tris, 1 M NaCl, pH 7.4
Biological Activity: Human HGF is fully biologically active when compared to standards. The activity was assayed for scattering activity in the MDCK cell assay. The ED50 was for this effect is typically at 0.5-1.0 ng/ml.
Reconstitution: The lyophilized human HGF is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in water to a concentration of 100 µg/ml. Further dilutions should be made into buffer containing protein or medium containing serum.
Stability: The lyophilized HGF, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted should be stored in working aliquots at-20°C to -70°C. Avoid repeated freeze-thaw cycles.
Usage: Human HGF is offered for in vitro research use-not for use in or on humans or animals.
Catalogue number: RDI-300-010 Size: 5 µg $190.00
RDI-300-011 Size: 25ug $688.00
Bulk material quoted on request
Literature: [Fausto N. J Hepatol 32; 19-31, 2000; Lamszus K et al. Int J Dev Neurosci 17; 517-530, 1999; Stella MC and Comoglio PM. Int J Biochem Cell Biol 31; 1357-1367, 1999]
** please note: always centrifuge vials before opening **
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