rev: October 19, 2004
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Human Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user to comply
with all local/state and Federal rules in the use of this product. We are
not responsible for any patent infringements that might result with the use
of or derivation of this product.
Endothelial-Monocyte Activating Polypeptide-II (EMAP-II) is a novel cytokine
that is an antiangiogenic factor in tumor vascular development, and strongly
inhibits tumor growth. Recombinant human EMAP-II is an 18.3 kDa protein
containing 166 amino acid residues.
Catalog#: RDI-1038
Package Size: 20 micrograms $231.00 $219.00/pack 2+
Mini-Pack: cat#RDI-1038p $94.00/5ug
-inquire for custom sizes/bulk
packaging
Supplied: Lyophilized powder from with no additives.
Source: E. Coli
Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than
0.1ng per ug (1EU/ug) of protein
Reconstitution: Lyophilized EMAP-II should be reconstituted in water to a
concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C,
mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm
to concentrate material in vial. This solution can be diluted into other
buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C
for future use. -Note:If this material is not to be used for coating or labeling
(or other applications requiring carrier free material), the addition of
0.1% BSA or HSA to dilution buffers or dilution in sterile culture media
is recommended to enhance stability and minimize absorption to vial. Ideally,
maintain stock >20ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks
but it is best stored desiccated at -20 DEG C. Reconstituted EMAP-II should
be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW
CYCLES.
Reactivity: Determined by the apoptotic effect on MCF-7 cells (human breast
carcinoma cells) using a concentration of 20-40 ng/ml.
AA Sequence :
SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP
RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM
VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP
KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM
SNSGIK
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.
cat#RDI-EMAP2abGP $212.00/50ug
cat#RDI-EMAP2abGP1 $300.00/100ug
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEMAP-II.
Anti-EMAP-II specific antibody was purified by affinity chromatography employing
immobilized hEMAP-II matrix.
anti-hEMAP-II:Biotin conjugated
-cat#RDI-EMAP2abGPB $212.00/25ug
cat#RDI-EMAP2abGPB1 $300.00/50ug
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEMAP-II
. Anti-hEMAP-2 specific antibody was purified by affinity chromatography and then
biotinylated.
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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