rev:   October 18, 2004


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET:   recombinant Human Keratinocyte Growth Factor (KGF)

-Keratinocyte Growth Factor (KGF or FGF-7) is a potent mitogen for keratinocytes and epithelial cells. Human KGF is an 18.9 kda protein containing 163 amino acid residues.

Catalog#: RDI-1019   Package Size: 10 micrograms $231.00    $219.00/pack 2 or more

                                   MINI-PACK cat#RDI-1019p $94.00/2ug
                                    -inquire for custom sizes/bulk packaging

Supplied: sterile filtered and the the protein was lyophilized from a 1.5X PBS pH7.0(no additives)

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of KGF.

Reconstitution: Reconstituted KGF is most stable at concentrations above 0.5mg/ml in solutions containing 30mM to 400mM NaCl, therefore it is recommended to reconstitute the lyophilized KGF in PBS to a concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for future use. Avoid freeze thaw cycles which will result in some loss of activity.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >1mg/ml.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted human KGF should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES. EACh FREEZE THAW CYCLE WILL DIMINISH ACTIVITY.

Reactivity: The ED50 as determined by the dose dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells is <10ng/ml, corresponding to a specific activity of  >100K units/mg.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049


phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266


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