rev: October 19, 2004
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Prolactin, also known as mammotrophin, luterotropic hormone, and lutetropin, is a neuroendocrine hormone secreted by the pituitary gland. Its primary function is to promote and maintain lactation during pregnancy and suckling. In addition, Prolactin plays an immune-regulatory role by stimulating the activities of ornithine decarboxylase and protein kinase C, which are important for the proliferation, differentiation and function of lymphocytes. This recombinant Human Prolactin is a 23 kDa globular protein containing 200 amino acid residues.
Catalog#: RDI-1007 lot#
Package Size: 50 micrograms $235.00 $219.00/pack 2+
Mini-pack:cat#RDI-1007P $94.00/10ug
-inquire for custom sizes/bulk packaging
Supplied: Lyophilized protein from 10mM sodium Phosphate pH8.0 + 50mM NaCl
with no additives.
Source: E. Coli
Purity: >95% by SDS-Page analysis. Endotoxin level is less than 0.1ng
per ug (1EU/ug) of human Prolactin.
Reconstitution: The lyophilized Prolactin is soluble in water and most aqueous
buffers. The lyophilized powder should be reconstituted in water, to a
concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG
C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material
in vial. This solution can be diluted into water or other buffered solutions
or stored at -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C. Reconstituted material should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
Reactivity: Determined by its ability to induce the proliferation of rat Nb2-11 cells in the concentration range of 0.1-1.0 ng/ml.
AA Sequence :
MLPICPGGAA RCQVTLRDLF DRAVVLSHYI HNLSSEMFSE FDKRYTHGRG FITKAINSCH TSSLATPEDK EQAQQMNQKD FLSLIVSILR SWNEPLYHLV TEVRGMQEAP EAILSKAVEI EEQTKRLLEG MELIVSQVHP ETKENEIYPV WSGLPSLQMA DEESRLSAYY NLLHCLRRDS HKIDNYLKLL KCRIIHNNNC
Precautions: For In vitro research Use Only. Not for use continued on back in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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