rev:  October 19, 2004


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

-Inquire for Bulk material

Recombinant Human Platelet Derived Growth Factor-AB

Description: Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human  PDGF-AB is a 25.5 kDa homodimer protein consisting of 13.3 kDa A chain and 12.2 B chain.

Cat: RDI-1000

Quantity: 10 ug (lyophilized)

Price: $231.00/package   $219.00/package 2+

          Mini-Pack: cat#RDI-1000p $94.00/2ug      

         -inquire for custom sizes/bulk packaging

Source: E. coli

Formulation: Lyophilized protein with no additives.

Stability: The lyophilized PDGF-AB, though stable at room temperature, is best stored desiccated below 0'C. Reconstituted PDGF-AB should be stored in working aliquots at -20'C.

Purity: Greater than 97% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per ug of PDGF-AB.

Reconstitution: The lyophilized PDGF-AB is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in 10 mM acetic acid to a concentration of 1 ug/ul. Allow to set at least 30 minutes at 4 DEG C, Mix Well, If desired, centrifuge for 1-2 minutes at 1000rpm to concentrate material in vial. This solution can be diluted into a aqueous buffered solutions or stored at -20' for future use. For most in vitro applications, PDGF-AB exerts its biological activity in the concentration range of 0.2 to 10.0 ng/ml.|

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Biological Activity: RDI human PDGF-AB is fully biologically active when compared to standards. The ED(50) as determined by the dose-dependent stimulation of thymidine uptake by BALB-c 3T3 cells is 1 ng/ml, corresponding to a specific activity of 1 X 10(6) units/mg.

Usage: For research use only. Nof for use in diagnostic or  therapeutic procedures.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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