rev: 5/15/2008
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Cytokines, Growth Factors & Chemokines:
(Rat and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of
recombinant growth factors, cytokines and chemokines
and antibodies. Since no one antibody works best for all applications
(neutralization, blotting, ELISA, etc), we offer many different types of
antibodies to help solve this problem. Please inquire for other applications or
types of antibodies not listed below.
Recombinant Rat VEGF-C
Description: VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT -4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant rat VEGF-C contains 129 amino acids residues and was fused to a His tag (6x His) at the c terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.
Source: Insect cells
Molecular Weight: 18-24 kDa
Purity: > 90%, by SDS-PAGE and visualised by silver stain
Endotoxin level: < 0.1 ng per mg of VEGF-C
Stabilizer: BSA
Buffer: none
Formulation: lyophilized
Biological Activity: Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells. The ED50 for this effect is typically 200-300 ng/ml.
Reconstitution: The lyophilised VEGF-C is soluble in water and most aqueous buffers. The lyophilised VEGF-C should be reconstituted in sterile DI water, then diluted into sterile PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.
Stability: Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF-C should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Usage: VEGF-C is offered for research use. Not for drug use. Not for human use!
Catalogue number:
RDI-R20-014, new cat#: 30R-AV005x $346.50/5ug
RDI-R20-014X, new cat#: 30R-AV005 or RDI-R20-015 $855.75/20ug
Range: 200-400 ng/ml
Literature: [Joukov et al., EMBO J 15:290, 1996; Olofsson et al., Curr Opin Biotech 10:528, 1999 ; Kukk et al., Development 122:3829, 1996]
see also rat VEGF-C152S
-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.
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RDI Division of Fitzgerald Industries Intl
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266