rev:May 5, 1997
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
CAT# RDI-PB13500
Quantity Shipped: 1 vial of 100K units in 0.5 ml in PBS con taining 0.1%
BSA.
Concentration: 2 x 10(5)units/ml (0.04 mg/ml).
Buffer: Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin
(BSA).
Polyacrylamide Gel Electrophoresis: one band, Mr 14,000; >95% purity.
Source: cDNA obtained from rat leukocyte expressed in E.coli.
Specific Activity: 5 x 10(6) units/mg.
Assay Used to Measure Bioactivity: Interferon was titrated with the use of
the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti,
P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37,
755-758; Familletti,P. C., Rubinstein,S., and Pestka, S. (1981)" A Convenient
and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods
in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394].
In this antiviral assays for interferon about 1 unit/ml of interferon is
the quantity necessary to produce a cytopathic effect of 50%. As there is
no international Rat IFN-G standard units are determined with respect to
the international reference standard for mouse interferon gamma (mouse IFN-G)
provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon
Standards and General Abbreviations,inMethods in Enzymology (S. Pestka, ed.),
Academic Press, New York 119, 14-23]. Unit of activity measured on mouse
L929 cells with encephalomyocarditis virus (EMCV).
Shipping Temperature: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at
-70'C or below for retention of full activity. When thawing, the contents
of the tube should be apportioned in separate tubes so that freezing and
thawing is kept to a minimum. Dilute only in buffers containing protein such
as 0.1% bovine serum albumin (BSA).
-For In Vitro Research Use Only-
Recombinant Rat Interferon Gamma (Rat IFN-G), carrier-free.
CAT# RDI-PB13501 $375.00 $312.00/vial 3+
Quantity Shipped: 1 vial of 5 ug in 20 ul.
Concentration: 0.25 mg/ml (1.25 x 10(6) units/ml)
Polyacrylamide Gel Electrophoresis: one band, Mr 14,000; >95% purity..
Source: cDNA obtained from rat leukocytes expresses in E. coli.
Specific Activity: 5 x 10(6) units/mg.
Assay Used to Measure Bioactivity: Interferon was titrated with the use of
the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti,
P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37,
755-758; Familletti,P. C., Rubinstein,S., and Pestka, S. (1981)" A Convenient
and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods
in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394].
In this antiviral assays for interferon about 1 unit/ml of interferon is
the quantity necessary to produce a cytopathic effect of 50%. As there is
no international Rat IFN-y standard, units are determined with respect to
the international reference standard for mouse interferon gamma (mouse IFN-y)
provided by the National Institutes of Health [see Pestka,S. (1986)"Interferon
Standards and General Abbreviations, " in Methods in Enzymology (S.Pestka,
ed.), Academic Press, New York 119, 14-23]. Unit of activity measured on
mouse L929 cells with encephalomyocarditis virus (EMCV).
Shipping Temperature: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at
-70'C or below for retention of full activity. When thawing, the contents
of the tube should be apportioned in separate tubes so that freezing and
thawing is kept to a minimum.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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Return (murine/rat cytokine index)
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