rev: April 3, 2000
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
CAT# RDI-PB13100 $438.00/vial $375.00/vial 3+
(new cat# for old#RDI-RTIFNA-100KU
Quantity Shipped: 1 vial of 1 x 100K units
(lyophilized)
Concentration: 1.5 x 10(5) units/ml (after reconstitution with 1ml sterile
distiled water)
Buffer: 50mM Tris-HCL, 20mM citric acid, 150mM NaCl,pH7 with 200ug rat serum
albumin and 125mM trehalose.
Source: cDNA expressed in baculovirus
Polyacrylamide Gel Electrophoresis: one band, Mr 19,000; >95%
purity.
Specific Activity: 6.0 x 10(7) units/mg.
Assay Used to Measure Bioactivity: Interferon was titrated with the use of
the cytopathic effect inhibition assay as described [Rubinstein,S.,Familletti,
P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37,
755-758; Familletti,P. C., Rubinstein,S., and Pestka, S. (1981)" A Convenient
and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods
in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394].
In this antiviral assays for interferon about 1unit/ml of interferon is the
quantity necessary to produce a cytopathic effect of 50%. As there is no
international Rat IFN-alpha standard, the units are determined with respect
to the international reference standard for mouse interferon alpha A/Beta
(MuIFN-a/B) provided by the National nstitutes of Health [see Pestka,
S. (1986)"Interferon Standards and General Abbreviations, in Methods in
Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. Unit of
activity measured on rat epithelial-like (RATEC) cells wih VSV in the presence
of 2mM glutathione.
Shipping Temperature: Ice Packs
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at
-70'C or below for retention of full activity. When thawing, the contents
of the tube should be apportioned in separate tubes so that freezing and
thawing is kept to a minimum. Use polypropylene pipette tips and tubes when
diluting this interferon. Dilute only in buffers containing protein such
as 0.1% bovine serum albumin (BSA)
-For In Vitro Research Use Only-
Precautions: For In vitro research Use Only. Not for use in or on humans or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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