rev:  May 12, 2005


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

Recombinant Human Interferon Beta 1A (Hu-IFN-B) (mammalian)

CAT# RDI-PB11410     $375.00/vial 100KU     $312.00/vial 5+

       #RDI-PB11410X    $625.00/1 million Units

Quantity Shipped: 1 vial of 1 x 100K units in 0.1 ml.

Concentration: 1 x 10(6) units/ml

Buffer:   10mM Acetic acid

Mr 23,000;  >95% purity, one N-glycosylation site

Source: cDNA obtained from human fibroblast mRNA expressed in CHO CELLS

Accession number: V00534

Specific Activity:  3.99 X 10 E08 untis/mg

Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-aA) provided by the National Institutes of Health [see Pestka, S.(1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. Unit of activity measured on bovine MDBK cells with vesicular stomatitis virus (VSV).

Shipping Temperature: Wet ice (DO NOT FREEZE)

Physical State of Product During Shipping: Liquid

Special Conditions/Comments: DO NOT FREEZE. After receipt, this product should be kept at 2-8 DEG C. Tighten cap, centrifuge 1 minute 1000rpm to concentrate the contents on  the bottom of the tube. Dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).

-For In Vitro Research Use Only-

-For In Vitro Research Use OnlyPrecautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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Ordering terms

RDI Div of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049


phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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