rev: 5/15/2008
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Human Cytokine/Growth Factor Sample Spec sheets (subject to change without notice):
Recombinant Human Interferon Alpha 2 (Hu-IFN-a2; Hu-IFN-a2b)
CAT# RDI-PB11105, new cat#: 30R-AI048 $451.00
Quantity Shipped: 1 vial of 100K units in 100 ul.
Concentration: 1 x 10(6) units/ml (3 ug/ml).
Buffer: Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin
(BSA).
Polyacrylamide Gel Electrophoresis: one band, Mr 19,269; >95% purity.
Source: cDNA obtained from human leukocyte mRNA
expressed in E. coli.
Specific Activity: 3 x 10(8) units/mg.
Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described
[Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P. C., Rubinstein,S.,
and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in
Methods in Enzymology, Vol. 78 (S.Pestka,
ed.), Academic Press, New York, 387-394]. In this antiviral assays for
interferon about 1 unit/ml of interferon is the quantity necessary to produce a
cytopathic effect of 50%. The units are determined
with respect to the international reference standard for human interferon alpha
A (Hu-IFN-aA) provided by the National Institutes of
Health [see Pestka, S. (1986)"Interferon
Standards and General Abbreviations, in Methods in Enzymology
(S. Pestka, ed.), Academic Press,
Shipping Temperature: Dry ice
Special Conditions/Comments: After receipt, this product should be kept at
-70'C or below for retention of full activity. When thawing, the contents of
the tube should be apportioned in separate tubes so that freezing and thawing
is kept to a minimum. Dilute only in buffers containing protein such as 0.1%
bovine serum albumin (BSA).
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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