rev: 5/15/2008
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Human Cytokine/Growth Factor Sample Spec sheets (subject to change without notice):
Recombinant Human Interferon Alpha A (Hu-IFN-aA; Hu-IFN-a2a)
CAT# RDI-PB11100, new cat#: 30R-AI051 $275.00
Actual Shipped: 1 vial of 5 Million units in 0.104ml.
Concentration: 5.0 x 10(7) units/ml
Buffer: Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin
(BSA).
Polyacrylamide Gel Electrophoresis: one band, Mr 19,241; >95% purity.
Source: cDNA obtained from human leukocyte mRNA expressed in E.coli.
Specific Activity: 2 x 10(8) units/mg.
Assay Used to Measure Bioactivity: Interferon was titrated with the use of the
cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C.,
and Pestka, S. (1981) "Convenient Assay for Interferons," J.
Virol.37, 755-758; Familletti,P. C., Rubinstein,S., and Pestka, S. (1981)"
A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon,"
in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York,
387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon
is the quantity necessary to produce a cytopathic effect of 50%. The units are
determined with respect to the international reference standard for human
interferon alpha A (Hu-IFN-aA) provided by the National Institutes of Health
[see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in
Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].
Unit of activity measured on bovine MDBK cells with vesicular stomatitis
virus (VSV).
Shipping Temperature: Dry ice
Special Conditions/Comments: After receipt, this product should be kept at -70'C
or below for retention of full activity. When thawing, the contents of the tube
should be apportioned in separate tubes so that freezing and thawing is kept to
a minimum. Dilute only in buffers containing protein such as 0.1% bovine serum
albumin (BSA).
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Recombinant Human Interferon Alpha A, carrier-free
(Hu-IFN-aA; Hu-IFN-a2a).
CAT# RDI-PB11101, new cat#: 30R-AI049 $379.50/20ug
Quantity Shipped: 1 vial of 20 ug in 0.05 ml.
Concentration: 0.4 mg/ml
MW: 19,241; >95% purity.
Source: cDNA obtained from human leukocyte mRNA expressed in E.coli.
Human Gene: IFNA2
Specific Activity: 3.8 x 10(8) units/mg.
Assay Used to Measure Bioactivity: Interferon was titrated withthe use of the
cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti,
P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J.
Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)"
A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon,"
in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York,
387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon
is the quantity necessary to produce a cytopathic effect of 50%. The units are
determined with respect to the international reference standard for human
interferon alpha A (Hu- IFN-aA) provided by the National Institutes of Health
[see Pestka, S. (1986)"Interferon Standards and General Abbreviations,in Methods
in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. Unit of
activity measured on bovine MDBK cells with vesicular stomatitis virus
(VSV).
Shipping Temperature: ICE PACK
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at
-70'C or below for retention of full activity. When thawing, the contents of
the tube should be apportioned in separate tubes so that freezing and
thawing is kept to a minimum.
For In Vitro Research Use Only-not for sale or use in any commerical kit or for use in the preparation of any commerical antibody. Not for diagnostic or therapeutic use.
For In Vitro Research Use Only
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phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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