rev:March 18, 2001
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-see also new mouse INTERFERON ALPHA ELISA KIT cat#RDI-PB42100 $500.00/kit
Recombinant Mouse Interferon Alpha A (Mouse IFN-aA) ALPHA A
CAT# RDI-PB12100 $375.00/vial $344.00/vial 3+
(new cat# for old#RDI-MIFNA-100KU
Quantity Shipped: 1 vial of 1 x 100K units in 0.1ml
Concentration: 1 x 10(6) units/ml
Buffer: Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin
(BSA).
Source: Gene obtained from mouse DNA expressed in E. coli.
Polyacrylamide Gel Electrophoresis: one band, Mr 19,323; >95% purity.
Specific Activity: 4.3 x 10(6) units/mg.
Assay Used to Measure Bioactivity: Interferon was titrated with the use of
the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti,
P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37,
755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient
and RapidCytopathic Effect Inhibition Assay for Interferon," in Methods in
Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York,387-394]. In
this antiviral assays for interferon about 1 unit/ml of interferon is the
quantity necessary to produce a cytopathic effect of 50%. The units are
determined with respect to the international reference standard for mouse
interferon alpha A/Beta (Mu- IFN-a/B) provided by the National Institutes
of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations,
in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119,
14-23]. Unit of activity measured on mouse L929 cells with
encephalomyocarditis virus (EMCV).
Shipping Temperature: Ice Packs
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at
-70'C or below for retention of full activity. Whenthawing, the contents
of the tube should be apportioned in sep-arate tubes so that freezing and
thawing is kept to a minimum. Use polypropylene pipette tips and tubes when
diluting this interferon. Dilute only in buffers containing protein such
as 0.1% bovine serum albumin (BSA) -For In Vitro Research Use Only-
Bulk QUOTES ON REQUEST
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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