recombinant Human and Mouse sLYVE-1 available through Research Diagnostics Inc

rev: December 23, 2004

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RDI Division of Fitzgerald Industries Intl offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Recombinant Human sLYVE-1

Cat#RDI-S01028 $250.00/5ug

Cat#RDI-S01029 $531.00/25ug

Description: A DNA sequence encoding the extracellular domain of human LYVE-1 (Met1 - Gly232) was fused to a C-terminal His -tag (6xHis) and expressed in insect cells . Based on N-terminal sequence analysis, the primary structure of recombinant mature sLYVE-1 starts at Ser24. sLYVE-1 has a calculated monomeric molecular mass of about 25kDa but as a result of glycosylation, migrates at approximately 35 - 45 kDa under reducing conditions in SDS-PAGE.

LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule bindd both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

Source: Insect cells

Molecular Weight: ~35-45 kDa

Purity: > 95%, by SDS-PAGE and visualised by silver stain

Endotoxin level: < 0.1 ng per µg of sLYVE-1

Stabilizer: none

Buffer: none

Formulation: lyophilised

Reconstitution: The lyophilised sLYVE-1 is soluble in water and most aqueous buffers. The lyophilised sLYVE-1 should be reconstituted in PBS or medium to a concentration not lower than 50 µg/ml.

Stability: Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted sLYVE-1 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Usage: sLYVE-1 is offered for research use. Not for drug use. Not for human use!

Literature: [Carriera et al., Cancer Res 61:8079, 2001; Jackson DG Trends Cardiovasc Med 13:1, 2003; Sleeman et al., Microsc Res Tech 55:61, 2001 ; Mäkinen et al., EMBO J 20 : 4762, 2001]

** please note : always centrifuge vials before opening **

Recombinant Mouse sLYVE-1

Cat#RDI-S01026 $250.00/5ug

Cat#RDI-S01027 $531.00/25ug

Description: A DNA sequence encoding the extracellular domain of mouse LYVE-1 (Met1 - Gly228) was fused to a C-terminal His -tag (6xHis) and expressed in insect cells . Based on N-terminal sequence analysis, the primary structure of recombinant mature sLYVE-1 starts at Ala24. sLYVE-1 has a calculated monomeric molecular mass of about 25kDa but as a result of glycosylation, migrates at approximately 35 - 45 kDa under reducing conditions in SDS-PAGE.