rev: January 12, 2005
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Cytokines, Growth Factors & Chemokines:ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Sinceno one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
-see also human IL-4 ELISA kits) -For Elispot/bulk Elisa also see the Pelipairs(tm)
cat#RDI-M2514clb Human IL-4 Elispot kit $500.00
Mouse anti-human IL-4 (Protein A purified, mIgG2a lyophilized, no carrier)
Rabbit anti-human IL-4 (antigen aff pure, lyophilized, no carrier/no preservative)
Rabbit anti-human IL-4:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)
Mouse anti-human IL-4
Rabbit anti-murine IL-4 (antigen aff pure, lyophilized, no carrier/no preservative)
Rabbit anti-murine IL-4:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)
mouse IL-4 matched pairs: cat#RDI-MIL4-KT $656.00/1000 wells
Rabbit anti-rat IL-4 (antigen aff pure, lyophilized, no carrier/no preservative)
Rabbit anti-rat IL-4:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)
recombinant mouse IL-4 cat#RDI-2144 $235.00/10ug
recombinant rat IL-4 cat#RDI-4004 $235.00/10ug
-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of theseproducts. Price/specifications/availability subject to change without notice.
LOT# see batch sheet
Package Size: 1.0 milligram purified IgG in 1.0ml PBS Buffer, pH 7.4. Prepared from neat SHEEP serum by ammonium sulphate precipitation. Sterile filtered, NO PRESERVATIVES OR CARRIER PROTEINS ADDED.
Species: Sheep IgG (>90% IgG by SDS-Page).
Activity: Reacts with human IL-4 (natural and recombinant forms). There is no cross reactivity with human IL-1A or B, Il-2, Il-6 ,Il-8, TNFA or GM-CSF.
Application -Neutralization:20ug/ml of antibody will neutralize 100% of the activity of 10ng/ml of recombinant human IL-4 .-see actual batch for specific information.
-Western blotting: 1:10 to 1:50 dilution
-immunoprecipitation:1:10 to 1:100 can be used as the primary capture antibody followed by anti-sheep IgG or a similar quantity can be bound to Protein A.
-All titers must be detemined in assay
Storage: Store at -20 Deg C upon receipt. Avoid repeated freeze thaw cycles. A carrier protein (BSA) or serum should be added to diluted antibody to maintain the protein concentration at 1mg/ml or greater. This reduces antibody loss by blocking non specific binding to container surfaces.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.
Also available,2 monoclonals against human Il-4. see cat#RDI-M1633clb & RDI-M1632clb
Price: $344.00/vial $312.00/vial 2+
-also available PE labeled cat#RDI-RTIL4abm-PE $469.00/100ug
Package Size: 100 micrograms in 0.2ml (0.5mg/ml) 50mM Tris pH8.0 with
150mM NaCl and 0.1% sodium azide
Ig Isotype: mIgG1k
Antigen: CHO cell derived rec rat IL-4
Purification: Protein G of tcs
Reactivity: flow cytometry (approx approx 1ug/million cells)see ref#4
-immunofluorescence (intracellular cytokine flow cytometry)
-neutralizing (azide free material)
REF: -J. Immunol. 156:2406-2412
-J. Exp Med 182:335-344
-J. Clin Invest 96:1484-1489
also available bulk: cat#RDI-RTIL4abm-X $500.00/0.5mg (no carrier) in PBS
-no carrier/sterile filtered cat#RDI-RTIL4abm-XP $625.00/0.5mg
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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