INTERFERON GAMMA (IFN-G) antibodies available through Research Diagnostics Inc

rev: December 27, 2004

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Cytokines, Growth Factors & Chemokines:ANTIBODIES

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

INTERFERON GAMMA

-also see our ELISA and compact kit against human IFN-G

-ELISA against rat or mouse Interferon gamma

-also antibodies against interferon receptors

cat#RDI-M2533clb Human IFN-gamma Elispot kits $500.00

MOUSE MONOCLONAL ANTIBODY AGAINST HUMAN INTERFERON GAMMA (MMHG-1)

CAT# RDI-PB2150 $375.00/vial

Quantity Shipped: 1 vial of 500 ug in 500 ul.

Concentration: 1 mg/ml.

Buffer: Phosphate buffered saline (PBS) with 0.1% BSA

Isotype: IgG1

Bioactivity: Binds to and neutralized human interferon gamma.

Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-gamma) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387- 394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-g provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material if prepared specifically for effective neutralization of Hu-IFN-G.

Usage: Western blot; immunoprecipitation; immunohistochemistry; ELISA.

Shipping Temperature: Dry ice

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -70'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

-For In Vitro Research Use Only-

MOUSE MONOCLONAL ANTIBODY AGAINST HUMAN INTERFERON GAMMA (MMHG-2)

non-neutralizing

CAT# RDI-PB21550 $375.00/vial

Quantity Shipped: 100 ug in 0.184 ml

Concentration: 0.545 mg/ml

Buffer: Phosphate buffered saline (PBS) with 0.1% BSA

Isotype: IgG1

Bioactivity: binds to but does not neutralize human interferon gamma.

Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-a) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-a provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material binds Hu-IFN-y well, but does not neutralize Hu-IFN-G

Usage: Western blot; immunoprecipitation; immunohistochemistry; ELISA.

Shipping Temperature: Dry ice

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

RABBIT POLYCLONAL ANTIBODY AGAINST HUMAN INTERFERON GAMMA

CAT#: RDI-PB31500 $312.00/vial

Quantity Shipped: 1 vial of 2 x 10(4) units in 0.1 ml.

Bioactivity: 2 x 10(5) neutralization units/ml

Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon gamma (Hu-IFN-G) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-y provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material if prepared specifically for effective neutralization of Hu-IFN-G

Usage: Western blot; immunoprecipitation; immunohistochemistry; ELISA.

Shipping Temperature: Dry ice

Physical State of Product During Shipping: Frozen

-For In Vitro Research Use Only-

Rabbit anti-human IFN-Gamma (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-IFNGabrP $250.00/50ug

cat#RDI-IFNGabrP1 $375.00/100ug

Rabbit anti-human IFN-Gamma:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-IFNGabrPB $250.00/25ug

cat#RDI-IFNGabrPB1 $375.00/50ug

Rabbit anti-mouse IFN-Gamma(antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-MIFNGabrP $250.00/50ug

cat#RDI-MIFNGabrP1 $375.00/100ug

Rabbit anti-mouse IFN-Gamma:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-MIFNGabrPB $250.00/25ug

cat#RDI-MIFNGabrPB1 $375.00/50ug

Goat anti-RAT IFN-Gamma(antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-RTIFNGabGP $250.00/50ug

cat#RDI-RTIFNGabGP1 $375.00/100ug

Goat anti-RAT IFN-Gamma:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-RTIFNGabGPB $250.00/25ug

cat#RDI-RTIFNGabGPB1 $375.00/50ug

Matched pairs (includes coating antibody, biotin labeled detection antibody and a calibrated standard) sufficient for up to 1000 wells

human IFN-Gamma cat#RDI-IFNG-KT $656.00

mouse IFN-Gamma cat#RDI-MIFNG-KT $656.00

rat IFN-Gamma cat#RDI-RTIFNG-KT $656.00

RABBIT anti-HUMAN INTERFERON GAMMA (neutralization)

Catalog#: RDI-IFNGabr $438.00/vial

Package Size: 1 mg purified IgG (via ammonium sulfate precipitation) in 1.0ml of PBS buffer, pH 7.4. NO preservative or carrier protein added. Sterile filtered.

Species: rabbit

Activity: Reacts specifically with human natural and recombinant interferon gamma. approx 20ug/ml will neutralize 4ng/ml of recombinant human interferon gamma.

Storage: Store at -20 DEG C immediatley upon arrival. Aliquot and avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

It is the responsibility of the user to comply with all local/stateand Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

DATA SHEET: Mouse anti-HUMAN INTERFERON GAMMA (Coating)

Catalog#: RDI-IFNGabm-C $562.00/vial

Package Size: 500 ug purified IgG (via Protein A) in 0.5 ml of PBS buffer. NO preservative or carrier protein added. Sterile filtered.

Clone: NIM42

Species: mouse IgG1k

Activity: Reacts specifically with human natural and recombinant interferon gamma.

-IN western blot, use approx 5-10ug/ml.

-IN Elisa use as a coating antibody. Detecting ab=RDI-IFNGabm-D (suggest using Biotin conjugate, cat#RDI-IFNGabm-BT)

-neutralization:

Storage: Store at -20 DEG C immediatley upon arrival. Aliquot and avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

also available with azide (non sterile) Bulk Quotes on Request

cat#RDI-IFNGabm-42 clone NIB42 $469.00/0.5mg