rev:     October 17, 2004

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

FUSIN ANTISERA

Background:Fusin (CXCR4) (subject to patent by University of Pa) is a seven-transmembrane domain G-protein linked chemokine receptor. It has the highest degree of sequence homology with IL-8 receptors. Fusin is expressed on a wide variety of CD4+ and CD4- cells including those from spleen, brain and lung. The gene is located on chromosome 2 close to other chemokine receptor genes. The CXC chemokine, stromal cell-derived factor-1 (SDF-1), was recently identified as the ligand for Fusin . This molecule is strongly chemotactic for monocytes and lymphocytes. Messenger RNA for SDF-1 has been detected in a wide range of tissues and it has been suggested that it may have a role in immune surveillance or lymphocyte recirculation, rather than in inflammation. SDF-1 is highly conserved in human and mouse and studies on SDF-1 knockout mice reveal an essential function in development, as the mice show defects in B cell lymphopoiesis, bone marrow myelopoiesis and in the structure of the heart.

Fusin functions as a cofactor for infection with T-cell tropic strains of HIV-1. Immunoprecipitation of Fusin with the HIVgp120-CD4 complex suggests a direct mode of action for Fusin as a co-receptor. The monoclonal antibody (mAb), 12G5, is specific for Fusin. Some HIV-2 isolates can utilize Fusin as a receptor for CD4-independent infection. 12G5 blocks both CD4-independent infection by HIV-2, and CD4-dependent infection by certain T-cell tropic isolates of HIV-11-5 and does not cross-react with IL8RA (CXCR1), IL8RB (CXCR2), CCR1, CCR2, CCR3, CCR4 or CCR5 or the murine SDF-1 receptor.

REFERENCES

1. Loetscher, M., T. Geiser, T. O'Reilly, et al. 1994. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. J. Biol. Chem. 269: 232.

2. Simmons, G., D. Wilkinson, J.D. Reeves, et al. 1996. Primary, syncytium-inducing human immunodeficiency virus type 1 isolates are dual-tropic and most can use either Lestr or CCR5 as coreceptors for virus entry. J. Virol. 70: 8355.

3. Nagasawa, T., T. Nakajima, K. Tachibana, et al. 1996. Molecular cloning and characterization of a murine pre-B-cell growth-stimulating factor/stromal cell-derived factor 1 receptor, a murine homolog of the human immunodeficiency virus 1 entry coreceptor fusin. Proc. Natl. Acad. Sci. USA 93: 14726.

4. Endres, M.J., P.R. Clapham, M. Marsh, et al. 1996. CD4-independent infection by HIV-2 is mediated by fusin/CXCR4.  Cell 87: 745.

5. Feng, Y., C.C. Broder, P.E. Kennedy, et al. 1996. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G-protein-coupled receptor. Science 272:809.

Catalog#: RDI-FUSINabm           100ug with azide     $406.00/vial

                RDI-FUSINabm1X      500ug (no azide)     $875.00

                RDI-FUSINabm-BT     100 tests Biotin      $500.00/100T vial

                RDI-FUSINabm-PE 100 tests PE labeled    $625.00/100T

Package Size: in TRIS-HCL pH 8.0 with 0.1% sodium azide. No azide formulation in PBS. (sodium azide is toxic,dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)

Prep: purified  by tissue culture supernatant by Protein G

Species: mouse IgG2ak

CLONE: 12G5

Activity: Reacts with fusin (CXCR4), a seven transmembrane domain, G linked glycoprotein. Fbusin is expressed on a wide variety of CD4+ and CD4- cells including those from spleen, brain and lung. Fusin has been shown to function as a cofactor for infection with T-cell tropic strains of HIV-1 and also as a receptor for CD4 independent infection. Clone 12G5 has been reported to block CD4 independent infection by HIV-2 and CD4 independent infection by some T-cell tropic isolates of   HIV-1.

Uses: flow cytometry (using amplified 3 step detecton process with Strepatvidin:PE) and indirect immunofluorescence

         -blocking experiments (using azide free formulation)

References: 1) J. Bio Chem 269:232, 1994

                   2) J. Virol, 70:8355, 1996

                   3) Proc Natl Acad Sci USA 93:14726, 1996

                   4) Cell 87:745

                   5) Science 272:809

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only.

-copyright by owner
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

cat# RDI-RTFUSINabr   $438.00

Packaging: 200ug Purified rabbit immunoglobulins, lyophilized (Reconstitute to 1 mg/ml by adding 200 ul PBS; contains 0.1% sodium azide)

Immunogen: E. coli-expressed rat CXCR4(fusin)

Use: -0.5-1ug/ml with chemiluminescence detection in Western blot

        -reacts with mouse and rat Cross-reactivity to perforin of other species has not been determined.

Storage: 4°C for short term storage or -20°C in small aliquots for long term storage. Avoid repeated freeze and thaw.

Background: CXCR4 (fusin) is a members in the seven-transmembrane domain G-protein-coupled chemokine receptor family. The full-length cDNA was isolated from a human spleen cDNA library 1 . Its ligand is chemokine stroma-derived factor (SDF). CXCR4 has been shown to be the co-receptor for HIV-1 binding to CD4 through the viral envelope glycoprotein gp120 2 . In other cases, CXCR4 can function as the only receptor for the interaction of HIV-2 with CD4 and host cells 3 . A mouse monoclonal antibody (12G5) to CXCR4 has been shown to inhibit HIV infectivity and HIV-induced syncytium.

General ref: 1. Federsppiel, B. et al. (1993) Molecular cloning of the cDNA and chromosomal localization of the gene for a putative seven-transmembrane segment (7-TMS) receptor isolated form human spleen. Genomics 16 (3): 707-12

2. Nagasawa, T. et al. (1998) A novel CXC chemokine PBSF/SDF-1 and its receptor CXCR4: their functions in development, hematopoiesis and HIV. Semin Immunol 10 (3): 179-85

3. Mcknight, A. et al. (1997) Inhibition of Human Immunodeficiency Virus Fusion by a Monoclonal Antibody to a Coreceptor (CXCR4) Is both Cell Type and Virus Strain Dependent. J. Virol., 71 (2): 1692-1696

For Research Use Only

Goat anti- mouse to CxCR4 (affinity purified)   N-terminal - extracellular domain

Cat# RDI-MCXCR4abGX  $565.00

Presentation: 200ug of peptide affinity purified IgG in PBS at 1.0mg/ml with 1 mg/ml BSA and 0.1% sodium azide. Antibody in formulation without azide for neutralization studies is also available.

Host: Goat

Antigen: NH2-YSEEVGSGDYDSNKEPCFRDENVHFNR

Specificity: Binds to CXCR 4 receptor on mouse spleen leukocytes as determined by immunocytochemistry.

Applications:

     ELISA: 1:50,000

     Western Blotting titer 1:1000

      Immunocytochemistry 1:300 (mouse spleen)

Storage: Freeze at -80 °C. Stable for at least 3 years when stored at -80 °C. Avoid freeze/thaw cycles.

References: 1. Bieniasz et.al. 1997. Murine CxCR-4 is a functional coreceptor for T-cell tropic and dual tropic strains of human immunodeficiency virus type 1. J Virol. 71: 7097.

2. Heesen, M. etal. 1996. Cloning of the mouse fusin gene. homologue to a human HIV-1 cofactor. J Immunol. 157: 5455.

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      or (978) 371-6446 or (800) 370-2222

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EMAIL:antibodies@fitzgerald-fii.com

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