rev:     October 17, 2004

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


(CCR-5)  see monoclonal and polyclonal below

 inquire for other chemokine receptor polyclonal antibodies or see antibody list


MOUSE MONOCLONAL ANTIBODY TO: CD 195 (CCR5 N-TERMINAL EXTRACELLULAR  DOMAIN)

PRODUCT CODE             RDI-CBL700     $375.00

CLONE                             12D1

ISOTYPE                          IgG2a

SOURCE                           Tissue culture supernatent

PURIFICATION

METHOD                          Protein A affinity chromatography

SPECIFICITY               This antibody recognizes an epitope in the N-terminal extracellular domain of CCR5. It is specific for CCR5 when the receptor is expressed in transfectants, including human, rodent, avian and insect target cell lines. CCR5 is the major chemokine co-receptor for macrophage-tropic strains of HIV-1. CCR5 binds RANTES, MIP-1a and MIP-Ib as expressed on T cells and macrophages; expression concomitant with CD4 renders cells permissive to macrophage-tropic but not T cell tropic virus strains.

APPLICATIONS *Flow cytometric studies and cell sorting for CCR5 expression in infections by expression systems, including human, rodent, avian and insect cells.*Western blotting

REFERENCES            Doranz et al. Journal of Virology 71: 6305-6314 (1997)

LOT SPECIFIC DATA

PRESENTATION          The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available: FITC conjugated cat#RDI-CBL700FT $375.00/100T

Bulk Quotes on request

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Goat anti-human CCR5 (affinity purified)   Antiserum (affinity purified) to C-C Chemokine   Receptor 5 (human)

cat# RDI-CCR5NabgX    $562.00

Presentation: 200ug of peptide affinity purified IgG in PBS with 1 mg/ml BSA and 0.1% sodium azide.

Antigen: Synthetic peptide (NH2-YQVSSPIYDINYYTSEPCQKINVKQIAA) corresponding to the N-terminal sequence of human CCR5.

Specificity: Binds to CCR 5 receptor on human peripheral blood leukocytes as determined by immunocytochemistry.

Applications:

  ELISA 1:50000

  Western Blot 1:1000

   Immunocytochemistry: 1: 300 (pos control human peripheral blood)

    Immunohistochemistry 1: 300 (human spleen)

Background: The HIV receptor is composed of two molecules, the CD4 binding receptor and a co-receptor. The chemokine receptor CCR5 is one of the co-receptors used by primary isolates of HIV-1. Extracellular domains of CCR5 are important for HIV entry into the target cells.

Storage/Stability: Freeze at -80 °C. Stable for at least 3 years when stored at -80 °C. Avoid freeze/thaw cycles.

References:

1. A RANTES, MIP-1a, MIP-1b receptor as fusion cofactor for macrophage-tropic HIV-1: Alkhatib et al.; Science 272, 1955 (1996)

2. Muliple Extracellular Domains of CCR-5 contribute to human immunodeficiency virus type 1 entry and fusion: J.Virol. 71, 5003 (1997)

For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax      (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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