rev: 5/16/2008
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CYTOCHROME P450
NATIVE ENZYMES
HUMAN LIVER MICROSOMES
PRODUCT NUMBER : RDI-HLMICRO, new cat#: 30R-AM040 $346.50/10mg
sample batch specs:
P450 CONTENT = 5.2 nmol/ml
PROTEIN CONTENT = 35.3 mg/ml
SPECIFIC CONTENT = 0.146 nmol P450/mg protein
Microsomes were prepared from donor human liver (single subject) using conventional homogenization and centrifugation techniques. Liver microsomes are provided in a solution containing 10 mM potassium phosphate buffer (pH 7.4) and 250 mM sucrose. The sample exhibits a typical P450 ferrous carbonyl absorption spectrum with minimal P420 content. Individual P450 enzyme content is shown below:
CYP2C9, 70.9 pmol/mg; CYP4A11, 44.7 pmol/mg; CYP4F2, 119.8 pmol/mg; CYP3A4, 41/100,
CYP2E1, 121/100, and CYP2A6, 129/100, where 100 denotes the level of these enzymes in a
microsomal sample denoted as a "standard one". UC9606 liver microsomes convert nifedipine to its oxidized pyridine metabolite (a CYP3A4-promoted reaction) at a rate of 0.48 nmol/min/mg protein., laurate to 12-hydroxylaurate at a rate of 0.59 nmol/min/mg protein
¨ Storage Microsomes should be stored @ -80°C. Avoid repeated freeze-thawing cycles.
For In Vitro Research Use Only
WARNING-POTENTIAL BIOHAZARDOUS MATERIAL
All products that contain human derived material have been found to be non-reactive for Hepatitis B Surface Antigen, HIV 1 & 2 and HCV antibodies. Because no test method can offer complete assurance that human T-lympotropic virus type III/lymphadenopathy associated virus (HTLV-III/LAV), Hepatitis B virus, or other infectious agents are absent, this specimen/reagent should be handled at the Biosafety Level 2 as recommended for any potentially infectious human serum or blood specimen in the Center for Disease Control/National Institutes of Health manual "Biosafety in Microbiological and Biomedical Laboratories", 1984.
Biosafety Level 2 practices, equipment, and facilities are applicable to clinical, diagnostic, teaching, and other facilities in which work is done with the broad spectrum of indigenous moderate-risk agents present in the community and associated with human disease of varying severity. With good microbiological techniques, those agents can be used safely in activities conducted on the open bench, provided the potential for producing aerosols is low. Hepatitis B virus, the Salmonellae, and Toxoplasma spp. are representative of microorganisms assigned to this containment level. Primary hazards to personnel working with these agents may include accidental autoinoculation, ingestion, and skin or mucus membrane exposure to infectious materials. Procedures with high aerosol potential that may increase the risk of exposure of personnel must be conducted in primary containment equipment of devices.
Storage : Microsomes should be stored @ -80°C. Avoid repeated
freeze-thawing cycles
RDI Division of Fitzgerald Industries Intl
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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