rev:January 23, 2002
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& Related Antibodies
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Rabbit anti-human Cytochrome P450 CYP4F2
CAT# RDI-CYP4F2abr Price: $469.00/1mg vial
IgG Fraction, Lyophylized
Antiserum was developed in rabbits using purified human liver CYP4F2 as immunogen. The whole IgG fraction was purified from antiserum using caprylic acid/ammonium sulfate fractionation .Anti-human CYP4F2 IgG is provided as 1mg ( 10mg/ml solution in 100mM potassium phosphate buffer pH7.4, 250mM KCL and 2.5uM thimerosal added as a preservative or as a powder lyophilized from this buffer.
Specificity has been determining by Western blotting. Anti-human CYP4F2 IgG reacts mainly with its corresponding 53kDa immunogen in human liver microsomes. A slight cross-reaction with a 70Kda non-P450 protein is sometimes observed using certain liver specimens. DUE TO EXTENSIVE strcutural homology between CYP4F2 and other CYP4F 450's, reaction of anti-CYP4F2 with CYP4F3a, CYP4F3B, CYP4F11 and/or CYP4F12 should be expected. Cross reactivity with whole human liver homogenates or the homologous CYP4F proteins in animal liver microsomes has not been determined.
Purity of the antibody has been established by SDS-PAGE
run under denaturing conditions. Two protein bands with molecular weights
of 50 kDa and 25 kDa can be visualized by Coomassie blue staining, which
correspond to the heavy and light chains, respectively, of rabbit IgG.
Reconstitute by adding 0.5 ml of distilled water to one
vial of lyophilized IgG (1 mg). Rotate vial gently until powder dissolves.
Add 0.5 ml of neat glycerol to obtain a 50% glycerol solution containing
1 mg IgG/ml. Prior to reconstitution, store the product at 0-5°C. After
reconstitution, the solution can be stored at -20°C since the presence
of 50% glycerol will prevent freeze/thaw cycles.
Immunoreactivity of Anti-CYP4F2 IgG with human liver proteins.
Lane A = UC9410 Liver microsomes (10 µg)
Lane B = Purified CYP4A11 (0.05 µg)
Lane C = Purified CYP4F2 (0.1ug)
Lane D=HK31 Kideny microsomes (15ug)
Use for Western Blotting
1. For full size (15 x 15 cm) blots, add 200 µg rabbit anti-human CYP4F2 IgG to 15-20 ml of blocking solution (e.g., Blotto). This corresponds to 100 µl of the 1.0 mg IgG/ml solution prepared in 50% glycerol as described above. The amount can be scaled down proportionally with smaller size blots.
2. Incubate overnight at room temperature, and remove antibody solution (which can be stored at -20°C for re-use). After washing blot extensively with blocking solution to remove unbound CYP4F2 antibody, incubate with an anti-rabbit IgG conjugate of choice (e.g, anti-rabbit IgG-peroxidase, anti-rabbit IgG-alkaline phosphatase or anti-rabbit IgG-biotin), and stain accordingly.
-Titrate with each application/detection system.
Precaution: For in vitro research use only-not for use in or on humans or
animals-not for use in diagnostics. Not responsible for any patent infringements
with the use or derivation of these products.
See also antibodies against: CYP2A6, CYP2C9, CYP2E1,CYP2C8, CYP3A4 CYP2C19 and CYP450 reductase. Inquire for:pure native P450 enzymes
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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