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ANTI-CYTOCHROME P450
& Related Antibodies
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYP2E1
Rabbit anti-human Cytochrome P450 CYP2E1
CAT# RDI-CYP2E1abr
Price: $484.00/1mg vial
IgG Fraction, Lyophylized
Antiserum was developed in rabbits using purified human liver CYP2E1 as
immunogen. The whole IgG fraction was purified from antiserum using caprylic
acid/ammonium sulfate fractionation (McKinney & Parkinson, J Immunol
Methods 96:271-278, 1987). Anti-human CYP2E1 IgG is provided in a
lyophilized state after freeze-drying from 100 mM potassium phosphate buffer
(pH 7.4), 250 mM KCl, and 2.5 µM thimerosal (added as a preservative)
- Specificity and Purity
Specificity has been determining by Western blotting. Anti-human CYP2E1 IgG reacts with only the corresponding immunogen in human liver microsomes. The antibody also recognizes the homologous CYP2E proteins in rat, rabbit, and hamster liver microsomes. Specificity with whole liver homogenates or S-9 fractions has not been determined.
Purity of the antibody has been established by SDS-PAGE run under denaturing conditions. Two protein bands with molecular weights of 50 kDa and 25 kDa can be visualized by Coomassie blue staining, which correspond to the heavy and light chains, respectively, of rabbit IgG.
- Reconstitution and Storage
Reconstitute by adding 0.5 ml of distilled water to one vial of lyophilized IgG (1 mg). Rotate vial gently until powder dissolves. Add 0.5 ml of neat glycerol to obtain a 50% glycerol solution containing 1 mg IgG/ml. Prior to reconstitution, store the product at 0-5°C. After reconstitution, the solution can be stored at -20°C since the presence of 50% glycerol will prevent freeze/thaw cycles.
Immunoreactivity of Anti-CYP2E1 IgG with human liver proteins.
Lane A = UMi592 microsomes (10 µg)
Lane B = Purified CYP2E1 (0.1 µg)
Lane C = UC9208 microsomes (10 µg)
· Use for Western Blotting
1. For full size (15 x 15 cm) blots, add 100 µg rabbit anti-human CYP2E1 IgG to 15-20 ml of blocking solution (e.g., Blotto). This corresponds to 100 µl of the 1.0 mg IgG/ml solution prepared in 50% glycerol as described above. The amount can be scaled down proportionally with smaller size blots.
2. Incubate overnight at room temperature, and remove antibody solution (which can be stored at -20°C for re-use). After washing blot extensively with blocking solution to remove unbound CYP2E1 antibody, incubate with an anti-rabbit IgG conjugate of choice (e.g, anti-rabbit IgG-peroxidase, anti-rabbit IgG-alkaline phosphatase or anti-rabbit IgG-biotin), and stain accordingly.
Precaution: For in vitro research use only-not for use in or on humans or
animals-not for use in diagnostics. Not responsible for any patent
infringements with the use or derivation of these products.
See also antibodies against: CYP2A6, CYP2C9, CYP2E1,CYP2C8, CYP3A4 (in
production), CYP2C19 and CYP450 reductase. Inquire for:pure native P450 enzymes
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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