rev: 5/16/2008
HOME (index page)
Return ( alphabetical antibody page)
Return (cytochrome P450 data page)
ANTI-CYTOCHROME P450
&
Related
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYP2C9
Rabbit anti-human Cytochrome P450 CYP2C9
CAT# RDI-CYP2C9abr
Price: $484.00/1mg vial
Presentation: 1 milligram lyophilized
Anti-human CYP2C9 IgG is provided as a powder after lyophylization from 100 mM
potassium phosphate buffer (pH7.4), 150 mM KCl, and 2.5 μM thimerosal
(added as a preservative). The whole IgG fraction was purified from antiserum
using caprylic acid/ammonium sulfate fractionation.
Specificity and Purity:
Specificity has been determined by Western blotting. Anti-human CYP2C9 IgG
reacts primarily with CYP2C9 (56 kDa) but also recognizes CYP2C19 (50 kDa) in
human liver microsomes; cross-reaction with CYP2C8 (52 kDa) is minimal. The antibody also cross-reacts with the homologous CYP2C proteins
in rat, rabbit, and hamster liver microsomes. Specificity with whole
liver homogenates or S-9 fractions has not been determined. Antibody purity has
been established by SDS-PAGE run under denaturing conditions. Two protein bands
with molecular weights of 50 kDa and 25 kDa can be visualized by Coomassie blue
staining, which correspond to the heavy and light chains, respectively, of
rabbit IgG.
Reconstitution of Lyophylized Product and Storage:
Prior to reconstitution, store product at 0-5°C. Reconstitute by adding 1
ml of PBS/50% glycerol to one vial
of lyophilized IgG (1 mg) and mix vial gently until powder dissolves. After
reconstitution, the solution can
be stored at -20°C, as the presence of glycerol will prevent freeze/thaw
cycles. Anti-CYP2C9 IgG solutions
without glycerol should also be stored at -20°C but seldom subjected to freeze/thaw.
Sample western blot data using the rabbit anti-CYP2C9 antibody Immunoreactivity of Anti-CYP2C9 IgG with human liver proteins (MW CYP2C9 = 56,000 daltons)
Lane A = UN504 microsomes (10 µg)
Lane B = Purified CYP2C9 (0.1 µg)
Lane C = UC9303 microsomes (10 µg)
Lane D = UC9406 microsomes (10 µg)
Anti-CYP2C9 IgG Reactivity with Human Liver
Lane
Lane 2 = Purified CYP2C19 (0.1 μg)
Lane 3 = Purified CYP2C8 (0.1 μg)
Lane 4 = Purified CYP2C9 (0.1 μg)
Use for Western Blotting:
For full size (15 x 15 cm) blots, add 100μg rabbit anti-human CYP2C9
IgG to 15-20 ml of blocking
solution (e.g., PBS containing 5% non-fat dry milk). This corresponds to 100
μl of the 1.0 mg IgG/ml
solution prepared as described above. The amount can be reduced with smaller
size blots.
Incubate overnight at room temperature, and remove antibody solution. After
washing blot with blocking
solution to remove unbound CYP2C9 antibody, incubate with an anti-rabbit IgG
conjugate of choice (e.g,
anti-rabbit IgG-peroxidase, anti-rabbit IgG-alkaline phosphatase or anti-rabbit
IgG-biotin), and develop
accordingly.
Precaution: For in vitro research use only-not for use in or on humans or
animals-not for use in diagnostics. Not responsible for any patent
infringements with the use or derivation of these products.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
Return (cytochrome P450 data page)