rev: January 23, 2002
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& Related Antibodies
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
PRODUCT NUMBER RDI-CYP2C19abr $562.00/1mg vial
ANTI-HUMAN CYP2C19 IgG
Polyclonal Antibody Developed in Rabbits
IgG Fraction, Lyophylized
Antiserum was developed in rabbits using purified recombinant human CYP2C19
as immunogen. The whole IgG fraction was purified from antiserum using caprylic
acid/ammonium sulfate fractionation. Anti-CYP2C19 IgG is provided in a
lyophilized state after freeze-drying from 100 mM potassium phosphate buffer
(pH 7.4), 250 mM KCl, and 2.5 µM thimerosal (added as a preservative).
¨ Specificity and Purity
Specificity has been determining by Western blotting. Anti-human CYP2C19
IgG reacts primarily with its immunogen (CYP2C19; 51 kDa) as well as with
CYP2C9 (56 kDa) in human liver microsomes; cross-reaction with CYP2C8 (52
kDa) is minimal, however. Reactivity of anti-CYP2C19 with CYP2C proteins
in rat, rabbit, and hamster liver microsomes is unknown. Purity of the antibody
has been established by SDS-PAGE run under denaturing conditions. Two protein
bands with molecular weights of 50 kDa and 25 kDa can be visualized by Coomassie
blue staining, which correspond to the heavy and light chains, respectively,
of rabbit IgG.
¨ Reconstitution and Storage
Reconstitute by adding 0.5 ml of distilled water to one vial of lyophilized IgG (1 mg). Rotate vial gently until powder dissolves. Add 0.5 ml of neat glycerol to obtain a 50% glycerol solution containing 1 mg IgG/ml. Prior to reconstitution, store the product at 0-5°C. After reconstitution, the solution can be stored at -20°C since the presence of 50% glycerol will prevent freeze/thaw cycles.
¨ Use for Western Blotting
1. For full size (15 x 15 cm) blots, add 50-100 µg rabbit anti-human CYP2C19 IgG to 15-20 ml of blocking solution (e.g., Blotto). This corresponds to 50-100 µl of the 1.0 mg IgG/ml solution prepared in 50% glycerol as described above. The amount can be scaled down proportionately with smaller size blots.
2. Incubate overnight at room temperature, and remove antibody solution (which can be stored at -20°C for re-use). After washing blot extensively with blocking solution to remove unbound CYP2C19 antibody, incubate with an anti-rabbit IgG conjugate of choice (e.g, anti-rabbit IgG peroxidase, anti-rabbit IgG-alkaline phosphatase or anti-rabbit IgG-biotin), and stain accordingly.
For In Vitro Research Use Only
Sample Blotting Data:
2=CYP2C19 0.1ug Unasborbed tested with 150ug/20ml of antibody, (7.5ug/ml)
3=CYP2C8 0.1ug -absorbed antibody at 300ug/20ml (15ug/ml)
4=CYP2C9 0.1ug -titer for each detection sysem
5=UC9408 Liver Mix 20ug -MW CYP2C19= 50 kDa, CYP2C9=56 kDa, CYP2C8=51.5kDa
6=UC9411 Liver Mix 20ug
See also antibodies against: CYP2A6, CYP2C8, CYP2C9, CYP2E1, CYP3A4 (in production), CYP2C19M ,CYP450 reductase, CYP450 side chain cleavage.
for:pure natural P450 enzymes
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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