rev:   July 2, 2004

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!

see below multi-types S-100 antigen (see also new homodimer below)

-see also s100.pdf

Human S-100 Antigen (BB homodimer and alpha/beta heterodimer)

CAT#: RDI-TRK8S9-2H     $406/0.1mg   $750.00/0.5mg    $1250.00/1mg  

Package: Each vial contains lyophilized

Source: Human brain tissue serum. Blood sample from tissue donor was tested and found negative for HBsAg, HIV-1 ,HIV-2 and HCV antibodies.

Presentation: Lyophilized from DI water with 5mM 2- mercaptoethanol and 5mM EDTA. It is recommended that the product should be reconstituted in 1ml 5mM 2-mercaptoethanol solution.

Analysis: Purity more 98% according to SDS-PAGE (Native gel electrophoresis, 20% Page with 2mM EGTA, stain coomassie blue R-250) MW approx 21kda,

Storage: At 4 DEG C or below in lyophilized form. After reconstitution the solution should be aliquoted and stored at- 20'C. Avoid frequent freeze thaw cycles.

For In Vitro Research Use Only-Not for Use in or on human or animals-not for use in diagnostics

Background: S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21kda. In human brain tissue, S-100 protein is mainly presented by two isoforms, a Beta-Beta(BB) homodimer or S-100b and an Alpha-Beta (AB) heterodimer or S-100a. Becasue of its predomiant location in astrological cells, S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples may eb useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, in    diagnosis and prognosis of clinical outcome in acute stroke.

Warning: Prepared from human tissue; As no method can assure complete non-infectivity, material should be handled under Biosafety Level II (CDC) condition and treated as if capable of transmitting infection.

see also monoclonals against S100

cat#RDI-TRK8S92h   ( Human S-100 Protein BB homodimer)

$406.00/0.1mg  $750.00/0.5mg  $1250.00/1mg   $1000.00/mg 2+

cat#RDI-TRK8S92b   ( BOVINE S-100 Protein BB homodimer)

$406.00/0.1mg   $750.00/0.5mg   $1250.00/1mg    $1000.00/mg 2+

BATCH SHEET: Recombinant Bovine S100B

Catalog#: RDI-S100B-AG    Price: $220.00/vial

Packaging: 10 ug (lyophilized) from 10mM Tris-HCL, 1mM EGTA

Reconstitution: with 10ul distilled water. Allow to set at least 30 minutes, tighten cap, centrifuge to concentrate material in vial. Recommend aliquoting and store -20 DEG C or below. -if suitable recommend adding 0.1% BSA to dilution buffers to reduce non specific absorption.

Species: E. Coli derived

Description: This recombinant protein has been purified by HPLC. This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.

Allow 2 weeks for delivery:

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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