rev:    October 16, 2003

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!

Recombinant Human Cytokeratin 18

cat#RDI-PRO62017   $625.00/250ug

cat#RDI-PRO62217   $375.00/100ug

Description                 Recombinant human cytokeratin 18

Molecular Weight:       45,000 (determined by SDS electrophoresis)

                                     48,201 (calculated from sequence)

Source:                         E. Coli

Purity:                          Greater than 95% (determined by SDS gel electrophoresis and western blot)

Application:                 -Protein standard in 1D and 2D SDS

                                    -Immunoassays

                                    -Immunization

Isoelectric point:         Ph I 5.7

Reconstitution:         100uG: When reconstituted with 70ul distilled water (final volume 100ul),

                                 250ug: when reconstituted with 175ul distilled water (final volume 250ul)

                              concentration of cytokeratin 18 is 1mg/ml in 30mM Tris/HCL pH 8, 9.5M urea, 2mM DTT, 2mM E                                 DTA, 10mM methylammonium chloride

Storage:             At 2-8 DEG C (lyophilized) Store -20 DEG C after reconstitituion Avoid frequent freeze thaw cycles.

Ref: Eur J Cell Biol 47:300-319 (1988)

Eur J Cell Biol 72:122-132 (1997)

-reconstitution to filaments is performed by mixing equimolar amounts of cytokeratins of Type 1 and type II at concentrations of approx 0.5mg/ml, both dissolved in 9.5M urea buffer (as per above formulation) Protofilaments and filament complexes are obtainined by dialyzing the resulting polypeptide stepwise to a concentration of 4M urea and then to low salt concentration (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCL, pH 7.4).

see J. Cell Biol 101, 1826-1841 (1995) and J. Mol. Biol 197, 237-255 (1987)

For IN Vitro Research Use Only

Strictly for in vitro research use only-not for use in or on humans or animals-not for diagnostics

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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