rev: November 28, 2004
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ANTIGENS/Pure
Proteins
(for in vitro research use only)
RDI Division of Fitzgerald Industries Intl offers a wide line of antigens for use
in histochemistry, ELISA, and further manufacturing use. Please inquire for
other antigens or antibodies to use with these products. Please inquire for
Bulk quantities-significant discounts available!
Recombinant Human Vimentin
cat#RDI-PRO62015 $815.00/250ug or cat#RDI-PRO62215 $410.00/100ug
Description: Recombinant human vimentin (intermediate filament
polypeptide)
Molecular Weight: 53, 685 (calculated from sequence) 57,000 (determined by SDS-gel electrophoresis)
Source: human, recombinant, expressed in E Coli
Purity: Greater than 95% (determined by SDS gel electrophoresis)
Application: -Protein standard in 1D and 2D SD
-Immunization
Isoelectric Point: pHI 5.3
Quanity: 250 ug lyophilized in 9.5M urea, 10mM Tris-HCL (pH 7.5), 2mM EDTA,
10mM methyammonium chloride ( protein con 1mg/ml after reconstitution as
below)
Reconstitution: When reconstituted with 200ul distilled water ( final volume
250ul), will contain 10mM sodium phosphate pH 7.5, 6M UREA, 1mM EDTA, 2mM
DTT, with a protein concentration of 1mg/ml (according to Bradford protein
determination)
Storage: At 2-8 DEG C (lyophilized)
Store -20 DEG C after reconstitituion Avoid frequent freeze thaw cycles.
For IN Vitro Research Use Only
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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