rev: 5/15/2008
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ANTIGENS/Pure Proteins
(for in vitro research use only)
RDI Division of Fitzgerald Industries Intl offers a wide line of antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!
PRODUCT DATA SHEET
CYTOKERATIN NO 8 (human Recombinant)
CAT# RDI-PRO62013, new cat#: 30R-AC041 $656.25/250ug
cat# RDI-PRO62203 $438.00/100ug
Molecular Weight 53,532 (calculated from sequence)
50,000 (determined by SDS gel
electrophoresis)
Source E. Coli
Application Protein standard in 1D and 2D SDS- gel
electrophoresis
Immunoassay
Antigen for immunisation
Purity Greater than 95%; determ.by SDS-PAGE
Amount 250ug (lyoph.,salt balanced)
Isoelectric point pH I: 6.1
Reconstitution (250ug) when reconstituted with 175ul H20 bidest,sol. (final
vol. 250ul)will contain 10mM Tris/HCl pH 8.5, 9.5M urea, 2mM Na EDTA 1mM
DTT,with a protein conc. of 1mg/ml
Storage -at 4 Deg.C (lyoph.) -20 DEG C (diluted) Avoid frequent freeze thaw
cycles
References
Krauss S. and Franke W.W. Organization and sequence of the human gene
encoding cytokeratin 8. Gene 86:241-249 (1990)
Magin T.M., Bader B.L., Freudenmann, M. and Franke W.W: De novo formation of
cytokeratin filaments in calf lens cells and cytoplasts after transfection with
cDNAs or microinjection with mRNAs encoding human cytokeratins. Eur. J. Cell
Biol. 53, 333-348 (1990)
Strictly for in vitro research use only-not for use in or on humans or
animals-not for diagnostics
RDI Division of Fitzgerald Industries Intl
(Research Diagnostics Inc)
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
email: sales@researchd.com
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