rev: October 16, 2003

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!

CYTOKERATIN NO 8 (human Recombinant)

CAT# RDI-PRO62013        $625.00/250ug

 cat#    RDI-PRO62203         $438.00/100ug

 

Molecular Weight    53,532 (calculated from sequence)

                               50,000 (determined by SDS gel   electrophoresis)

Source E. Coli

Application        Protein standard in 1D and 2D SDS- gel electrophoresis

                          Immunoassay

                         Antigen for immunisation


Purity Greater than   95%; determ.by SDS-PAGE


Amount 250ug (lyoph.,salt balanced)

Isoelectric point pH I: 6.1

Reconstitution (250ug) when reconstituted with 175ul H20 bidest,sol. (final vol. 250ul)will contain 10mM Tris/HCl pH 8.5, 9.5M urea, 2mM Na EDTA 1mM DTT,with a protein conc. of 1mg/ml


Storage -at 4 Deg.C (lyoph.) -20 DEG C (diluted) Avoid frequent freeze thaw cycles

References

Krauss S. and Franke W.W. Organization and sequence of the human gene encoding cytokeratin 8. Gene 86:241-249 (1990)

Magin T.M., Bader B.L., Freudenmann, M. and Franke W.W: De novo formation of cytokeratin filaments in calf lens cells and cytoplasts after transfection with cDNAs or microinjection with mRNAs encoding human cytokeratins. Eur. J. Cell Biol. 53, 333-348 (1990)

Strictly for in vitro research use only-not for use in or on humans or animals-not for diagnostics

RDI Division of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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