rev: September 8, 1999

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


PRODUCT DATA SHEET

STANDARD: VINCULIN

CAT# RDI-PRO62012 (250ug)   PRICE: $375.00

Molecular Weight 130 000

Source Chicken gizzard

Application Protein standard in 1D and 2D SDS- gel electrophoresis Immunoassay

                  Antigen for immunisation

Purity Greater than 90%; determ. by SDS-PAGE


                                      a_____

1) myosin (a),                                  ____

    B-galactosidase (b),    b_____

    phosphorylas B (c),     c_____

    BSA (d),                     d_____

    ovalbumin (e);

2)  vinculin                         e___

                                             1           2


Amount 250ug (lyoph.,salt balanced)


ORDER NO. RDI-PRO62012  (250ug)


Isoelectric point pHl 7.0


Reconstitution (250ug) when reconstituted with 250ul H20 bidest,sol. (final vol. 250ul)will contain 20mM Tris/Acetate,pH 7.6, 20mM NaCl, 0.1mM EDTA, 2mM DTT,with a protein conc. of 1mg/ml(according to Bradford protein determination).


Storage at 4Deg.C (lyoph.)   at -20Deg.C (sol.)


References

Feramisco J.R. and Burridge, K.: A rapid purification of alpha-actinin, filamin, and a 130,000 dalton protein from smooth muscle. J.Biol Chem. 255, 1994 ff, (1980).

Geiger, B.: A. 130 kD protein from chicken gizzard: Its localiation at the termini of microfilament bundles in cultured chicken cells. Cell 18, 193 ff, (1979).



For In Vitro Research Use Only


RDI Division of Fitzgerald Industries Intl

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Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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