rev:  January 22, 2004

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!

PRODUCT DATA SHEET


STANDARD :NEUROFILAMENT


CAT# RDI-PROL62010 (250ug)    PRICE: $438.00


Molecular Weight 200 000

Source Bovine spinal cord

Application Protein standard in 1D and 2D SDS  -gel electrophoresis

                  Immunoassay

                 Antigen for immunization

Purity Greater than 98%; determ. by SDS-PAGE

             

1)  myosln (a),                             a _____       _____

     B-galactosidase  (b),                                                    ____

     phosphorylase B (c),               b _____

     BSA (d),                                 c _____

     ovalbumin (e);                          d _____                                   ____

2) Mr 200 kD Neurofilament;

3) Mr 160 kD Neurofilament;        e _____

4) Mr 69 kD Neurofilament

                                                           1               2           3             4


Quantity 250ug (lyoph.)


Isoelectric point pHl 5.5


Reconstitution (250ug) when reconstituted with 200ul H20 bidest, sol. (final vol. 250ul) will contain 10mM sodium phosphate pH 7.5, 2mM DDT, 6M urea, 1 mM EDTA, with a protein conc.of 1 mg/ml (according to Bradford protein determination).

Storage at 4Deg.C (lyoph.)   at -20Deg.C (sol.)


References

Dahl,D.,Crosby,C.J.,Gardner, E.E. and Bignami, A.: Purification of the glial fibrillary acidic protein by anion-exchange chromatography, Analyt, Biochem. 126, 165 ff. (1982)


Weber,K., Shaw,G.,Osborn, M.,Debus, E. and Geisler,N.:Neurofilaments, a subclass of intermediate filaments:Structure and expression.Cold Spring Harbor Symp. Quant.Biol. 48, 717 ff, (1983)



For In Vitro Research Use Only

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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