rev:  September 8, 1999

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!

PRODUCT DATA SHEET


STANDARD:  NEUROFILAMENT 68Kda


CAT# RDI-PRO62008 (250ug)   PRICE: $375.00

Molecular Weight 68 000

Source Bovine   spinal cord

Application Protein standard in 1D and 2D SDS- gel electrophoresis

                   Immunoassay

                  Antigen for immunization

Purity Greater than 98%; determination by SDS-PAGE


1)           myosin (a),                a_____     ____

              B-galactosidase (b),                               ___

              phosphorylas B (c),    b_____

              BSA (d),                    c_____

              ovalbumin (e);            d_____                           ____

2) Mr 200 kD Neurofilament;

3) Mr 160 kD Neurofilament;   e_____

4) Mr 68 kD Neurofilament

                                                    1             2           3       4


Quantity 250ug (lyoph.)


CAT. NO. RDI-PRO62008 (250ug)  $375.00

Isoelectric point pHl 5.0

Reconstitution (250ug) when reconstituted with 200ul H20 bidest, sol. (final vol. 250ul) will contain 10mM sodium phosphate, pH 7.5, 2mM DDT, 6M urea, 1mM EDTA, with a protein conc. of 1mg/ml (according to Bradfor protein determination).

Storage at 4Deg.C. (lyoph.)   at -20Deg.C (sol.)


References


Dahl,D.,Crosby, C.J. Gardner, E.E.and Bignami,A.: Purification of the glial fibrillary acidic protein by anion-exchange chromatography, Analyt. Biochem. 126, 165 ff, (1982).


Weber,K.,Shaw, G., Osborn, M., Debus, E. and Geisler, N.: Neurofilaments, a subclass of intermediate filaments: Structure and expression. Cold Spring Harbor Symp. Quant.Biol.48, 717 ff,(1983).


For In Vitro Research Use Only

RDI Division of Fitzgerald Industries Intl

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USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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