rev: 5/15/2008
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ANTIGENS/Pure Proteins
(for in vitro research use only)
RDI Division of Fitzgerald Industries Intl offers a wide line of antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!
PRODUCT DATA SHEET
STANDARD: FILAMIN
CAT# RDI-PRO62006, new cat#: 30R-AF016 PRICE: $412.50/250ug
Molecular Weight 250 000
Source Chicken gizzard
Application Protein standard in 1D and 2D SDS- gel electrophoresis
Immunoassay
Antigen for
immunisation
Purity Greater than 90%; determ. by SDS-PAGE
1) filamin; ____
2) myosin (a), ____a
B-galactosidase (b), ____b
phosphorylase B (c), ____c
BSA (d), ____d
ovalbumin (e)
____e
1 2
Amount 250ug (lyoph., salt balanced)
ORDER NO:RDI-PRO62006 (250ug)
Isoelectric point pHl 6.4
Reconstitution (250ug) when reconstituted with 250ul Laemmli buffer or 9 M urea
(eg IEF- or NEPHGE buffer),sol.(final vol. 250ug) will additionally contain
20mM Tris/Acetate pH .6,20mM NaCl, 0.1mM EDTA, 2mM DTT,with a protein conc. of
1 mg/ml (according to Bradford protein determination).
Storage at 4Deg.C (lyoph.) at -20Deg.C )sol.)
References
Feramisco J.R. and Burridge,K.: A rapid purification of alpha-actinin, filamin, and a 130,000 dalton protein from smooth muscle J. Biol Chem, 255, 1194 ff, (1980)
Davies,P.J.A.,Wallach,D.,Willingham,M.,Pestan, I.and Lewis,
M.S.:Self-association of chicken gizzard filamin and heavy merofilamin,Biochem,
19, 365 ff, (1984)
For In Vitro Research Use Only
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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