rev: 5/15/2008
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ANTIGENS/Pure Proteins
(for in vitro research use only)
RDI Division of Fitzgerald Industries Intl offers a wide line of antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!
PRODUCT DATA SHEET
STANDARD : a-ACTININ
: CAT# RDI-PRO62002, new cat#: 30R-AA001 $484.00/250ug
Molecular Weight 100 000
Source Chicken gizzard
Application: Protein standard in 1D and 2D SDS-gel electrophoresis
Immunoassay
Antigen for immunisation
Purity Greater than 95%; determ. by SDS-PAGE
1) a-actin; _____ a
2) myosin (a),
B-galactosidase (b), _____ b
phosphorylase B (c), ___
_____ c
BSA (d), _____ d
ovalbumin (e)
_____ e
1 2
Amount 250ug (lyoph.,salt balanced)
ORDER NO:RDI-CBL62002 (250ug)
Isoelectric point pHl 5.8
Reconstitution (250ug) when reconstituted with 250ul H20 bidest,vol.(final vol.250ul) will contain 20mM Tris/Acetate, pH 7.6,0.1 mM EDTA, 2mM DTT, 20mM NaCl, with a protein conc. of 1mg/ml (according to Bradford protein det.).
Storage at 4Deg.C (lyoph.) at -20Deg.C (sol.)
Reference:Duhaiman, A.S. and Bamburg.J.R.:Isolation of brain alpha-actinin.1:s characterization and a comparison of its properties with those of muscle alpha-actinins.Biochem, 23, 1600 ff,(1934)
Feramisco,J.R.and Burridge, K.:A rapid purification of a-actinin,filamin and a 130,000 dalton protein from smooth muscle,J.Biol.Chem. 255, 1194 ff, (1980).
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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